CXCL14/BRAK Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-31398

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Bovine (100%), Porcine (100%), Rat (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Validated:

Multiplex Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CXCL14/BRAK Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CXCL14/BRAK. The exact sequence is proprietary.

Localization

Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

13 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit CXCL14/BRAK Antibody - BSA Free (NBP1-31398) is a polyclonal antibody validated for use in Multiplex Immunofluorescence, IHC, WB and ICC/IF. Anti-CXCL14/BRAK Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CXCL14/BRAK Antibody - BSA Free

Immunohistochemistry: CXCL14/BRAK Antibody [NBP1-31398]

Immunohistochemistry: CXCL14/BRAK Antibody [NBP1-31398]

CXCL14-BRAK-Antibody-Immunohistochemistry-NBP1-31398-img0013.jpg
Western Blot: CXCL14/BRAK Antibody [NBP1-31398]

Western Blot: CXCL14/BRAK Antibody [NBP1-31398]

Western Blot: CXCL14/BRAK Antibody [NBP1-31398] - Human tissue extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with CXCL14 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Frozen: CXCL14/BRAK Antibody [NBP1-31398]

Immunohistochemistry-Frozen: CXCL14/BRAK Antibody [NBP1-31398]

Immunohistochemistry-Frozen: CXCL14/BRAK Antibody [NBP1-31398] - Human lung tissue stained for CXCL14 (red) counterstained with DAPI. IHC-Fr image submitted by a verified customer review.
Immunohistochemistry-Paraffin: CXCL14/BRAK Antibody [NBP1-31398]

Immunohistochemistry-Paraffin: CXCL14/BRAK Antibody [NBP1-31398]

Immunohistochemistry-Paraffin: CXCL14/BRAK Antibody [NBP1-31398] - Human renal cell carcinoma, using CXCL14 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: CXCL14/BRAK Antibody [NBP1-31398]

Immunohistochemistry-Paraffin: CXCL14/BRAK Antibody [NBP1-31398]

Immunohistochemistry-Paraffin: CXCL14/BRAK Antibody [NBP1-31398] - Human renal cell carcinoma, using CXCL14 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry: CXCL14/BRAK Antibody [NBP1-31398]

Immunohistochemistry: CXCL14/BRAK Antibody [NBP1-31398]

CXCL14-BRAK-Antibody-Immunohistochemistry-NBP1-31398-img0012.jpg
CXCL14/BRAK Antibody

Immunocytochemistry/ Immunofluorescence: CXCL14/BRAK Antibody [NBP1-31398] -

Immunocytochemistry/ Immunofluorescence: CXCL14/BRAK Antibody [NBP1-31398] - Two-color immunofluorescence staining of primary colon cancer tissue with anti-CXCL14 & BerEP4, & anti-CXCL16 & BerEP4. (A) Anti-CXCL14, (E) Anti-CXCL16 both red color. (B,F) BerEP4 mAb, green color. (C,G) Overlays giving yellow color of double-stained areas. (D) FITC-conjugated mouse IgG; negative control for BerEP4. (H) Rabbit IgG; negative control for anti-CXCL14 & anti-CXCL16. Original magnification: ×200. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31752131), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for CXCL14/BRAK Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Reported in scientific literature (PMID: 28775895).

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

Validated from a verified customer review.

Immunohistochemistry-Paraffin

1:100-1:1000

Knockdown Validated

Reported in scientific literature (PMID: 28775895).

Western Blot

1:500-1:3000

Reviewed Applications

Read 2 reviews rated 4.5 using NBP1-31398 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CXCL14/BRAK

The CXCL14 gene belongs to the cytokine gene family which encode secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC (Cys-X-Cys) subfamily of cytokines. Members of this s

Alternate Names

Bolekine, BRAK, CXC-X3

Gene Symbol

CXCL14

Additional CXCL14/BRAK Products

Product Documents for CXCL14/BRAK Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CXCL14/BRAK Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Chemokines and Receptors

Citations for CXCL14/BRAK Antibody - BSA Free

Customer Reviews for CXCL14/BRAK Antibody - BSA Free (2)

4.5 out of 5
2 Customer Ratings
5 Stars
50%
4 Stars
50%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used CXCL14/BRAK Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 2 of 2 reviews Showing All
Filter By:
Clear Filters X
  • CXCL14/BRAK Antibody
    Name: Jan Martinek
    Application: Immunohistochemistry-Frozen
    Sample Tested: human lung tissue
    Species: Human
    Verified Customer | Posted 07/27/2021
    Human lung stained for CXCL14 (red) counterstained with DAPI.
    PFA fixed fresh frozen sample
    CXCL14/BRAK Antibody - BSA Free NBP1-31398
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Human
    Species: Human
    Verified Customer | Posted 12/28/2011

There are no reviews that match your criteria.

Showing  1 - 2 of 2 reviews Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies