CXCR3 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-56404

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Proximity Ligation Assay

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Proximity Ligation Assay, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CXCR3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a synthetic peptide corresponding to amino acids 290-303 of human CXCR3.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CXCR3 Antibody - BSA Free

Western Blot: CXCR3 Antibody [NB100-56404]

Western Blot: CXCR3 Antibody [NB100-56404]

Western Blot: CXCR3 Antibody [NB100-56404] - HEK293T cells. Western blot image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: CXCR3 Antibody [NB100-56404]

Immunocytochemistry/ Immunofluorescence: CXCR3 Antibody [NB100-56404]

Immunocytochemistry/Immunofluorescence: CXCR3 Antibody [NB100-56404] - Brain microvascular endothelial cells isolated from WT and CXCR3 KO mice were stained with anti-CXCR3 (green) and anti-ZO-1 (blue) antibodies to analyze CXCR3 expression by immunofluorescence. Image from verified customer review.
Immunohistochemistry-Paraffin: CXCR3 Antibody [NB100-56404]

Immunohistochemistry-Paraffin: CXCR3 Antibody [NB100-56404]

Immunohistochemistry-Paraffin: CXCR3 Antibody [NB100-56404] - IHC analysis of a FFPE human spleen using 1:25 dilution of CXCR3 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of HIER using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (40X) was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.
Western Blot: CXCR3 Antibody [NB100-56404]

Western Blot: CXCR3 Antibody [NB100-56404]

Western Blot: CXCR3 Antibody [NB100-56404] - Analysis of CXCR3 in human pancreas lysate using CXCR3 antibody at 2 ug/mL. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Applications for CXCR3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 31887215)

Immunohistochemistry-Frozen

reported in scientific literature (PMID 19234157)

Immunohistochemistry-Paraffin

1:10 - 1:500

Proximity Ligation Assay

reported in scientific literature (PMID 34433033)

Western Blot

1 - 3 ug/mL
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reviewed Applications

Read 2 reviews rated 5 using NB100-56404 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CXCR3

Chemokines play important roles in inflammation and critical for the recruitment of effector immune cells to sites of infection. Historically, CD183 was the third CXC chemokine receptor discovered and therefore, commonly designated as CXCR3. Binding of chemokines to CD183 induces cellular responses that are involved in leukocyte traffic, most notably integrin activation, cytoskeletal changes and chemotactic migration. The CXC chemokine receptor (CXCR) 3, a G-protein-coupled 7-transmembrane receptor, is expressed at high levels on activated and memory T cells, B cells, natural killer cells, and plasmacytoid monocytes.

Alternate Names

CD183, CXCR3, GPR9

Entrez Gene IDs

2833 (Human); 12766 (Mouse)

Gene Symbol

CXCR3

UniProt

P49682

Additional CXCR3 Products

Product Documents for CXCR3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CXCR3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CXCR3 Antibody - BSA Free

Customer Reviews for CXCR3 Antibody - BSA Free (2)

5 out of 5
2 Customer Ratings
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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • CXCR3 Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Brain microvascular endothelial cells
    Species: Mouse
    Verified Customer | Posted 01/18/2023
    Brain microvascular endothelial cells isolated from WT and CXCR3 KO mice were stained with anti-CXCR3 (green) and anti-ZO-1 (blue) antibodies to analyze CXCR3 expression by immunofluorescence.
    CXCR3 Antibody - BSA Free NB100-56404
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: 293T lysate
    Species: Human
    Verified Customer | Posted 09/11/2014
    Western blot for CXCR3 in 293T cells
    CXCR3 Antibody - BSA Free NB100-56404

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CXCR3 Antibody - BSA Free

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  • Q: Regarding recommended dilutions the manual contains: "Immunohistochemistry-Paraffin, Western Blot 1-3 ug/ml" Does it mean 1-3 ug/ml is recommended for both applications or there is simply no recommended dilution IHC-P?

    A: For our product NB100-56404, we did not have a dilution recommendation listed for IHC on our datasheet. I am sorry for any confusion. This application was added to our product based on a publication that actually uses this product at 1:200 in paraformaldehyde fixed frozen tissue samples: NCBI site.

  • Q: Which isoform of CXCR3 is this antibody specific for?

    A: NB100-56404 can only detect human CXCR3 isoforms 1 and 2 in WB and IHC-Fr, the validated applications.

  • Q: Regarding recommended dilutions the manual contains: "Immunohistochemistry-Paraffin, Western Blot 1-3 ug/ml" Does it mean 1-3 ug/ml is recommended for both applications or there is simply no recommended dilution IHC-P?

    A: For our product NB100-56404, we did not have a dilution recommendation listed for IHC on our datasheet. I am sorry for any confusion. This application was added to our product based on a publication that actually uses this product at 1:200 in paraformaldehyde fixed frozen tissue samples: NCBI site.

  • Q: Which isoform of CXCR3 is this antibody specific for?

    A: NB100-56404 can only detect human CXCR3 isoforms 1 and 2 in WB and IHC-Fr, the validated applications.

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