CYBB/NOX2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-41291

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CYBB/NOX2 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 15 amino acid peptide near the amino terminus of human NOX2. The immunogen is located within amino acids 140 - 190 of NOX2. Amino Acid Squence: NFARKRIKNPEGGLY

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Porcine (87%), Bovine (87%)

Specificity

At least two isoforms are known to exist; this antibody will detect both isoforms. NOX2 is predicted to not cross-react with other NOX proteins.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CYBB/NOX2 Antibody - BSA Free

Western Blot: CYBB/NOX2 AntibodyBSA Free [NBP2-41291]

Western Blot: CYBB/NOX2 AntibodyBSA Free [NBP2-41291]

Western Blot: CYBB/NOX2 Antibody [NBP2-41291] - Analysis of NOX2 in rat brain tissue lysate with NOX2 antibody at 1 ug/ml.
Immunohistochemistry: CYBB/NOX2 Antibody - BSA Free [NBP2-41291]

Immunohistochemistry: CYBB/NOX2 Antibody - BSA Free [NBP2-41291]

Immunohistochemistry: CYBB/NOX2 Antibody [NBP2-41291] - NOX2 expression in mouse cerebral cortex. Dilution 1:1000. This image was submitted via customer Review.
CYBB/NOX2 Antibody - BSA Free

Immunohistochemistry: CYBB/NOX2 Antibody - BSA Free [NBP2-41291] -

Immunohistochemistry: CYBB/NOX2 Antibody - BSA Free [NBP2-41291] - Immunohistochemistry of CYBB/NOX2 in rat brain tissue cells with CYBB/NOX2 antibody at 2 ug/ml.
CYBB/NOX2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: CYBB/NOX2 Antibody - BSA Free [NBP2-41291] -

Immunocytochemistry/ Immunofluorescence: CYBB/NOX2 Antibody - BSA Free [NBP2-41291] - Immunofluorescence of CYBB/NOX2 in rat brain tissue with CYBB/NOX2 antibody at 20 u/ml.

Green: CYBB/NOX2 Antibody
Blue: DAPI staining
CYBB/NOX2 Antibody - BSA Free

Immunohistochemistry: CYBB/NOX2 Antibody - BSA Free [NBP2-41291] -

Immunohistochemistry: CYBB/NOX2 Antibody - BSA Free [NBP2-41291] - Immunohistochemistry of CYBB/NOX2 in rat brain tissue with CYBB/NOX2 antibody at 5 ug/ml.
CYBB/NOX2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: CYBB/NOX2 Antibody - BSA Free [NBP2-41291] -

Immunocytochemistry/ Immunofluorescence: CYBB/NOX2 Antibody - BSA Free [NBP2-41291] - Immunofluorescence of CYBB/NOX2 in rat brain tissue with CYBB/NOX2 antibody at 20 ug/ml.
CYBB/NOX2 Antibody - BSA Free

Western Blot: CYBB/NOX2 Antibody - BSA Free [NBP2-41291] -

Exogenous 8-OHdG decreases NOX1/2/4 expression and NF-kappa B phosphorylation, and increases the reduced glutathione/oxidized glutathione ratio in DOX-treated H9c2 cells. A Rac1 activation assay. After H9c2 cells were treated with DOX (1 μM) and 8-OHdG (100 or 250 μg/mL) for 1 h, cell lysates were precipitated by p21-activated protein kinase (PAK) p21-binding domain (PBD) agarose beads and immunoblotted by Rac1 specific monoclonal antibody. B–D H9c2 cells were treated with 1 μM DOX and 100 μg/mL or 250 μg/mL 8-OHdG for 24 h. B–C Western blot analysis of the NOX1/2/4, p65, and phosphor-p65 protein levels of in DOX- and 8-OHdG-treated H9c2 cells. D GSH/GSSG ratio was determined using a glutathione assay kit. CTL, control; DOX, doxorubicin; 8-OHdG, 8-hydroxydeoxyguanosine; NOX, NADPH oxidase; GSH/GSSG, reduced glutathione/oxidized glutathione ratio. *P < 0.05 versus control; #P < 0.05 and ##P < 0.01 versus DOX-treated groups Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36517746), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
CYBB/NOX2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: CYBB/NOX2 Antibody - BSA Free [NBP2-41291] -

Immunofluorescence analysis of NOX-2 in experimental groups (A–L). The nuclei were labeled with DAPI (blue). NOX-2 expression was similar in the control, APO, and MSG + APO groups. An increased number of NOX-2-positive cells (red) were observed in the MSG group. White arrows indicate NOX-2-positive cells. Scale bar: 50 μm. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/2075-1729/13/3/822), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for CYBB/NOX2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

2 ug/ml

Immunohistochemistry-Paraffin

2 ug/ml

Western Blot

1-2 ug/ml

Reviewed Applications

Read 1 review rated 4 using NBP2-41291 in the following applications:

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CYBB/NOX2

Long Name

Cytochrome b-245 heavy chain

Alternate Names

CGD91-phox, CYBB, EC 1.-.-.-, gp91-1, gp91-phox, NADPH oxidase 2, NOX2

Gene Symbol

CYBB

Additional CYBB/NOX2 Products

Product Documents for CYBB/NOX2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CYBB/NOX2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CYBB/NOX2 Antibody - BSA Free

Customer Reviews for CYBB/NOX2 Antibody - BSA Free (1)

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Customer Images

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  • CYBB/NOX2 Antibody
    Name: Hayk Simonyan
    Application: Immunohistochemistry
    Sample Tested: Fixe mouse brain tissue, Mouse brain and Brain (cortex) tissue
    Species: Mouse
    Verified Customer | Posted 05/16/2017
    IHC image showing NOX2 expression in mouse cerebral cortex. Dilution 1:1000.
    CYBB/NOX2 Antibody - BSA Free NBP2-41291

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