CYC1 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-86872
![Immunocytochemistry/ Immunofluorescence: CYC1 Antibody [NBP1-86872]](https://resources.rndsystems.com/images/products/CYC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86872-img0011.jpg "Immunocytochemistry/ Immunofluorescence: CYC1 Antibody [NBP1-86872]")
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Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Immunohistochemistry-Paraffin, Western Blot, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: NSEAARAANNGALPPDLSYIVRARHGGEDYVFSLLTGYCEPPTGVSLREGLYFNPYFPGQAIAMAPPIYTDVLEFDDGTPATMSQIAKDVCTFLRWASEPEHDHRKRMGLKMLMMMALLVPLVYTIKRHKWSVLKSRKLAYR
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CYC1 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: CYC1 Antibody [NBP1-86872]
Immunocytochemistry/Immunofluorescence: CYC1 Antibody [NBP1-86872] - Staining of human cell line U-251 MG shows localization to mitochondria. Antibody staining is shown in green.![Immunohistochemistry: CYC1 Antibody [NBP1-86872]](https://resources.rndsystems.com/images/products/CYC1-Antibody-Immunohistochemistry-NBP1-86872-img0018.jpg "Immunohistochemistry: CYC1 Antibody [NBP1-86872]")
Immunohistochemistry: CYC1 Antibody [NBP1-86872]
CYC1-Antibody-Immunohistochemistry-NBP1-86872-img0018.jpg![Immunohistochemistry-Paraffin: CYC1 Antibody [NBP1-86872]](https://resources.rndsystems.com/images/products/CYC1-Antibody-Immunohistochemistry-Paraffin-NBP1-86872-img0014.jpg "Immunohistochemistry-Paraffin: CYC1 Antibody [NBP1-86872]")
Immunohistochemistry-Paraffin: CYC1 Antibody [NBP1-86872]
Immunohistochemistry-Paraffin: CYC1 Antibody [NBP1-86872] - Staining of human cerebral cortex shows moderate granular positivity in cytoplasm in neurons.![Immunohistochemistry-Paraffin: CYC1 Antibody [NBP1-86872]](https://resources.rndsystems.com/images/products/CYC1-Antibody-Immunohistochemistry-Paraffin-NBP1-86872-img0016.jpg "Immunohistochemistry-Paraffin: CYC1 Antibody [NBP1-86872]")
Immunohistochemistry-Paraffin: CYC1 Antibody [NBP1-86872]
Immunohistochemistry-Paraffin: CYC1 Antibody [NBP1-86872] - Staining of human kidney shows strong granular positivity in cytoplasm in cells in tubules.![Immunohistochemistry-Paraffin: CYC1 Antibody [NBP1-86872]](https://resources.rndsystems.com/images/products/CYC1-Antibody-Immunohistochemistry-Paraffin-NBP1-86872-img0017.jpg "Immunohistochemistry-Paraffin: CYC1 Antibody [NBP1-86872]")
Immunohistochemistry-Paraffin: CYC1 Antibody [NBP1-86872]
Immunohistochemistry-Paraffin: CYC1 Antibody [NBP1-86872] - Staining of human skeletal muscle shows moderate granular positivity in cytoplasm in myocytes.![Western Blot: CYC1 Antibody [NBP1-86872]](https://resources.rndsystems.com/images/products/CYC1-Antibody-Western-Blot-NBP1-86872-img0013.jpg "Western Blot: CYC1 Antibody [NBP1-86872]")
![CYC1 Antibody - BSA Free Western Blot: CYC1 Antibody - BSA Free [NBP1-86872]](https://resources.rndsystems.com/images/products/nbp1-86872_rabbit-polyclonal-cyc1-antibody-304202513105820.jpg "Western Blot: CYC1 Antibody - BSA Free [NBP1-86872]")
Western Blot: CYC1 Antibody - BSA Free [NBP1-86872]
Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry: CYC1 Antibody - BSA Free [NBP1-86872] -
Tissue localization and semi-quantification of the mitochondrial respiratory enzyme complexes in the murine distal colon during C. rodentium infection.Immunohistochemical staining using antibodies for (A) MTND6 (complex I) (B) SDHA (complex-II) (C) CYC1 (complex-III) (D) CCO-VIc (complex-IV). Statistics: unpaired t test. *P < 0.05, **P < 0.01, ***P < 0.001 vs. control, n = 5–6 mice/group. The infection experiments were performed twice, and each time point contain results pooled from 5–6 mice. Scale bar 100 μm, magnification × 200. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26481427), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for CYC1 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Reviewed Applications
Read 1 review rated 4 using NBP1-86872 in the following applications:
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CYC1
Alternate Names
Complex III subunit 4, Complex III subunit IV, cytochrome c1, heme protein, mitochondrial, cytochrome c-1Cytochrome b-c1 complex subunit 4, Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit, UQCR4
Gene Symbol
CYC1
Additional CYC1 Products
Product Documents for CYC1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CYC1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for CYC1 Antibody - BSA Free
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Application: ImmunocytochemistrySample Tested: MCF-7Species: RatVerified Customer | Posted 12/07/2015MCF-7 mitochondria GFP tagged cells were treated CYC1 inhibitor
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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