Cyclin D1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-16054

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Rat, Canine, Feline

Predicted:

Bovine (93%), Canine (94%), Mouse (93%), Rhesus Macaque (99%). Backed by our 100% Guarantee.

Applications

Knockout Validated, Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Cyclin D1 Primary Antibodies »

Product Specifications

Immunogen

Full length human Cyclin D1 Recombinant protein.

Reactivity Notes

Chicken (86%), Xenopus laevis (80%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cyclin D1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Cyclin D1 Antibody [NBP2-16054]

Immunocytochemistry/ Immunofluorescence: Cyclin D1 Antibody [NBP2-16054]

Immunocytochemistry/Immunofluorescence: Cyclin D1 Antibody [NBP2-16054] - SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cyclin D1 protein stained by Cyclin D1 antibody [N1C3] diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [11710] diluted at 1:500. Scale bar = 10 um.
Immunoprecipitation: Cyclin D1 Antibody [NBP2-16054]

Immunoprecipitation: Cyclin D1 Antibody [NBP2-16054]

Immunoprecipitation: Cyclin D1 Antibody [NBP2-16054] - Immunoprecipitation of Cyclin D1 protein from MCF-7 whole cell extracts using 5 ug of Cyclin D1 antibody [N1C3]. Western blot analysis was performed using Cyclin D1 antibody [N1C3]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
ELISA: Cyclin D1 Antibody [NBP2-16054]

ELISA: Cyclin D1 Antibody [NBP2-16054]

ELISA: Cyclin D1 Antibody [NBP2-16054] - Detection of recombinant full-length Cyclin D1 protein using as capture antibody at concentration of 5 ug/mL and as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) (NBP2-19301) was diluted at 1:10000 and used to detect the primary antibody.
Cyclin D1 Antibody

Western Blot: Cyclin D1 Antibody [NBP2-16054] -

Western Blot: Cyclin D1 Antibody [NBP2-16054] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody (NBP2-16054) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Cyclin D1 Antibody

Immunohistochemistry-Paraffin: Cyclin D1 Antibody [NBP2-16054] -

Immunohistochemistry-Paraffin: Cyclin D1 Antibody [NBP2-16054] - Cyclin D1 antibody detects Cyclin D1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded cat liver.
Cyclin D1 stained by Cyclin D1 antibody (NBP2-16054) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Cyclin D1 Antibody

Western Blot: Cyclin D1 Antibody [NBP2-16054] -

Western Blot: Cyclin D1 Antibody [NBP2-16054] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody [N1C3] (NBP2-16054) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Cyclin D1 Antibody

Western Blot: Cyclin D1 Antibody [NBP2-16054] -

Western Blot: Cyclin D1 Antibody [NBP2-16054] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody (NBP2-16054) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Cyclin D1 Antibody

Western Blot: Cyclin D1 Antibody [NBP2-16054] -

Western Blot: Cyclin D1 Antibody [NBP2-16054] - Wild-type (WT) and Cyclin D1 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Cyclin D1 Antibody - BSA Free

Western Blot: Cyclin D1 Antibody [NBP2-16054] -

Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody [N1C3] (NBP2-16054) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Cyclin D1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:1000-1:10000

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Cyclin D1

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq]

Alternate Names

Bcl-1, CCND1, Cyl-1, PRAD1

Gene Symbol

CCND1

UniProt

P24385

Additional Cyclin D1 Products

Product Documents for Cyclin D1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Cyclin D1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Cyclin D1 Antibody - BSA Free

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Protocols

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