Cycloheximide

Tocris Bioscience | Catalog # 0970

Inhibitor of protein synthesis
Tocris Bioscience

Key Product Details

Description

Inhibitor of protein synthesis
View all DNA, RNA and Protein Synthesis Inhibitors »

Product Description

Cycloheximide is a selective inhibitor of eukaryotic (over prokaryotic) protein synthesis, blocking tRNA binding and release from ribosomes. Induces apoptosis in a variety of transformed and normal cell lines, including T cells. Competitively inhibits the PPIase hFKBP12 (Ki = 3.4 μM). Also inhibits ferroptosis. Antifungal antibiotic. Exhibits anti-MERS-CoV activity in Vero cells in vitro (IC50 = 0.16 μM).

Product Specifications for Cycloheximide

Molecular Weight

281.35

Formula

C15H23NO4

Storage

Desiccate at RT

Purity

≥97% (HPLC)

Chemical Name

4-[2-(3,5-Dimethyl-2-oxo-cyclohexyl)-2-hydroxyethyl]-2,6-piperidinedione

CAS Number

66-81-9

PubChem ID

6197

InChI Key

YPHMISFOHDHNIV-FSZOTQKASA-N

SMILES

O=C(N1)CC(C[C@@H](O)[C@]2([H])C[C@@H](C)C[C@H](C)C2=O)CC1=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 7.03 25
Ethanol 14.07 50

Preparing Stock Solutions for Cycloheximide

The following data is based on the product molecular weight 281.35.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 7.11 mL 35.54 mL 71.09 mL
2.5 mM 1.42 mL 7.11 mL 14.22 mL
5 mM 0.71 mL 3.55 mL 7.11 mL
25 mM 0.14 mL 0.71 mL 1.42 mL

Calculators

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Product Documents for Cycloheximide

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Cycloheximide

For research use only

⚠ WARNING: This product can expose you to chemicals including Cycloheximide, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Customer Reviews for Cycloheximide (3)

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3 Customer Ratings
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Showing  1 - 3 of 3 reviews Showing All
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  • CHX
    Name: P Kumar
    Species: Rat
    Assay Type: In Vitro
    Cell Line/Tissue: H9C2
    Verified Customer | Posted 05/28/2021
    Treated H9C2 to inhibit protein translation.
    Cycloheximide 0970
  • complete inhibition of protein synthesis
    Name: Paramananda Saikia
    Species: Mouse
    Assay Type: In Vitro
    Cell Line/Tissue: AML12
    Verified Customer | Posted 01/11/2018
    required dilution made fresh.
    20 microgram/ml for 5 hours
  • protein synthesis inhibitor
    Name: Winnie Hui
    Species: Human
    Verified Customer | Posted 01/04/2018
    Product is easy to resuspend and works well with experiments

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