Cyclophilin-E Antibody (2F5) - Azide and BSA Free

Novus Biologicals | Catalog # H00010450-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2F5

Format

Azide and BSA Free
View all Cyclophilin-E Primary Antibodies »

Product Specifications

Immunogen

PPIE (NP_006103, 2 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ATTKRVLYVGGLAEEVDDKVLHAAFIPFGDITDIQIPLDYETEKHRGFAFVEFELAEDAAAAIDNMNESELFGRTIRVNLAKPMRIKEGSSRPVWSDDD

Specificity

PPIE - peptidylprolyl isomerase E (cyclophilin E)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Cyclophilin-E Antibody (2F5) - Azide and BSA Free

Western Blot: Cyclophilin-E Antibody (2F5) [H00010450-M02]

Western Blot: Cyclophilin-E Antibody (2F5) [H00010450-M02]

Western Blot: Cyclophilin-E Antibody (2F5) [H00010450-M02] - PPIE monoclonal antibody (M02), clone 2F5. Analysis of PPIE expression in NIH/3T3.
Immunocytochemistry/ Immunofluorescence: Cyclophilin-E Antibody (2F5) [H00010450-M02]

Immunocytochemistry/ Immunofluorescence: Cyclophilin-E Antibody (2F5) [H00010450-M02]

Immunocytochemistry/Immunofluorescence: Cyclophilin-E Antibody (2F5) [H00010450-M02] - Analysis of monoclonal antibody to PPIE on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: Cyclophilin-E Antibody (2F5) [H00010450-M02]

Western Blot: Cyclophilin-E Antibody (2F5) [H00010450-M02]

Western Blot: Cyclophilin-E Antibody (2F5) [H00010450-M02] - PPIE monoclonal antibody (M02), clone 2F5. Analysis of PPIE expression in PC-12.
Western Blot: Cyclophilin-E Antibody (2F5) [H00010450-M02]

Western Blot: Cyclophilin-E Antibody (2F5) [H00010450-M02]

Western Blot: Cyclophilin-E Antibody (2F5) [H00010450-M02] - PPIE monoclonal antibody (M02), clone 2F5 Analysis of PPIE expression in Hela S3 NE.
Western Blot: Cyclophilin-E Antibody (2F5) [H00010450-M02]

Western Blot: Cyclophilin-E Antibody (2F5) [H00010450-M02]

Western Blot: Cyclophilin-E Antibody (2F5) [H00010450-M02] - PPIE monoclonal antibody (M02), clone 2F5. Analysis of PPIE expression in Raw 264.7.
Western Blot: Cyclophilin-E Antibody (2F5) [H00010450-M02]

Western Blot: Cyclophilin-E Antibody (2F5) [H00010450-M02]

Western Blot: Cyclophilin-E Antibody (2F5) [H00010450-M02] - Analysis of PPIE expression in transfected 293T cell line by PPIE monoclonal antibody (M02), clone 2F5.Lane 1: PPIE transfected lysate(33.4 KDa).Lane 2: Non-transfected lysate.
ELISA: Cyclophilin-E Antibody (2F5) [H00010450-M02]

ELISA: Cyclophilin-E Antibody (2F5) [H00010450-M02]

ELISA: Cyclophilin-E Antibody (2F5) [H00010450-M02] - Detection limit for recombinant GST tagged PPIE is approximately 0.1ng/ml as a capture antibody.

Applications for Cyclophilin-E Antibody (2F5) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate, transfected lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Cyclophilin-E

The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein contains a highly conserved cyclophilin (CYP) domain as well as an RNA-binding domain. It was shown to possess PPIase and protein folding activities and also exhibit RNA-binding activity. Three alternatively spliced transcript variants encoding distinct isoforms have been observed.

Alternate Names

Cyclophilin E, Cyclophilin-33, CYP33, CYP-33, EC 5.2.1.8, MGC111222, MGC3736, peptidyl-prolyl cis-trans isomerase E, peptidylprolyl isomerase E (cyclophilin E), PPIase E, Rotamase E

Entrez Gene IDs

10450 (Human)

Gene Symbol

PPIE

OMIM

602435 (Human)

UniProt

NP_006103 (Human)

Additional Cyclophilin-E Products

Product Documents for Cyclophilin-E Antibody (2F5) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Cyclophilin-E Antibody (2F5) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Cyclophilin-E Antibody (2F5) - Azide and BSA Free

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Protocols

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FAQs

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