Cyclophilin-F Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-15079

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (90%), Chicken (95%), Rhesus Macaque (99%). Backed by our 100% Guarantee.

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Cyclophilin-F Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cyclophilin-F. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (82%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cyclophilin-F Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Cyclophilin-F Antibody [NBP2-15079]

Immunocytochemistry/ Immunofluorescence: Cyclophilin-F Antibody [NBP2-15079]

Immunocytochemistry/Immunofluorescence: Cyclophilin-F Antibody [NBP2-15079] - Analysis of methanol-fixed A431, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: Cyclophilin-F Antibody [NBP2-15079]

Immunohistochemistry-Paraffin: Cyclophilin-F Antibody [NBP2-15079]

Immunohistochemistry-Paraffin: Cyclophilin-F Antibody [NBP2-15079] - Human colon carcinoma, using PPIF antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: Cyclophilin-F Antibody [NBP2-15079]

Western Blot: Cyclophilin-F Antibody [NBP2-15079]

Western Blot: Cyclophilin-F Antibody [NBP2-15079] - ouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with PPIF antibody [N1C3] diluted at 1:5000.
Knockout Validated: Cyclophilin-F Antibody [NBP2-15079]

Western Blot: Cyclophilin-F Antibody [NBP2-15079]

Western Blot: Cyclophilin-F Antibody [NBP2-15079] - Wild-type (WT) and Cyclophilin F knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cyclophilin-F antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Cyclophilin-F Antibody

Western Blot: Cyclophilin-F Antibody [NBP2-15079] -

Western Blot: Cyclophilin-F Antibody [NBP2-15079] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Cyclophilin-F antibody (NBP2-15079) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Cyclophilin-F Antibody

Western Blot: Cyclophilin-F Antibody [NBP2-15079] -

Western Blot: Cyclophilin-F Antibody [NBP2-15079] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Cyclophilin-F antibody (NBP2-15079) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Cyclophilin-F Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Cyclophilin-F

The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death. [provided by RefSeq]

Alternate Names

Cyclophilin F, CYP3FLJ90798, Cyp-D, hCyP3, MGC117207, mitochondrial, peptidyl-prolyl cis-trans isomerase, mitochondrial, peptidylprolyl isomerase F, peptidylprolyl isomerase F (cyclophilin F)

Gene Symbol

PPIF

UniProt

P30405 (Human)

Additional Cyclophilin-F Products

Product Documents for Cyclophilin-F Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Cyclophilin-F Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for Cyclophilin-F Antibody - BSA Free

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