CYP27A1 Antibody - Azide Free

Novus Biologicals | Catalog # NBP2-16062

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free
View all CYP27A1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human CYP27A1. The exact sequence is proprietary.

Localization

Mitochondrion membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CYP27A1 Antibody - Azide Free

Western Blot: CYP27A1 Antibody [NBP2-16062]

Western Blot: CYP27A1 Antibody [NBP2-16062]

Western Blot: CYP27A1 Antibody [NBP2-16062] - Sample (50 ug of whole cell lysate) A: Mouse Liver. Antibody at 1:1000.
Immunohistochemistry-Paraffin: CYP27A1 Antibody [NBP2-16062]

Immunohistochemistry-Paraffin: CYP27A1 Antibody [NBP2-16062]

Immunohistochemistry-Paraffin: CYP27A1 Antibody [NBP2-16062] - CYP27A1 antibody detects CYP27A1 protein at cytoplasm in human endometrial carcinoma by immunohistochemical analysis. CYP27A1 antibody at 1:500.
Western Blot: CYP27A1 Antibody [NBP2-16062]

Western Blot: CYP27A1 Antibody [NBP2-16062]

Western Blot: CYP27A1 Antibody [NBP2-16062] - Sample (30 ug of whole cell lysate) A: A549. Separation by 7.5% SDS-PAGE. Antibody at 1:1000.
Western Blot: CYP27A1 Antibody [NBP2-16062]

Western Blot: CYP27A1 Antibody [NBP2-16062]

Western Blot: CYP27A1 Antibody [NBP2-16062] - A. 50 ug rat liver lysate/extract.
Immunohistochemistry-Paraffin: CYP27A1 Antibody [NBP2-16062]

Immunohistochemistry-Paraffin: CYP27A1 Antibody [NBP2-16062]

Immunohistochemistry-Paraffin: CYP27A1 Antibody [NBP2-16062] - U87 xenograft, using CYP27A1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
CYP27A1 Antibody

Western Blot: CYP27A1 Antibody [NBP2-16062] -

Western Blot: CYP27A1 Antibody [NBP2-16062] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYP27A1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Applications for CYP27A1 Antibody - Azide Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100 - 1:1000

Western Blot

1:500 - 1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS

Format

Azide Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CYP27A1

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein oxidizes cholesterol intermediates as part of the bile synthesis pathway. Since the conversion of cholesterol to bile acids is the major route for removing cholesterol from the body, this protein is important for overall cholesterol homeostasis. Mutations in this gene cause cerebrotendinous xanthomatosis, a rare autosomal recessive lipid storage disease. [provided by RefSeq]

Long Name

Sterol 26-hydroxylase, mitochondrial

Alternate Names

CYP27, Cytochrome P450 27, EC 1.14.15.15

Gene Symbol

CYP27A1

UniProt

Q02318 (Human)

Additional CYP27A1 Products

Product Documents for CYP27A1 Antibody - Azide Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CYP27A1 Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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