CYP4V2 Antibody (M29-P3B10) - BSA Free
Novus Biologicals | Catalog # NBP2-50214
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # M29-P3B10
Format
BSA Free
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Product Specifications
Immunogen
Peptide sequence - KREELGLEGQ (amino acids 495 - 504)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for CYP4V2 Antibody (M29-P3B10) - BSA Free
Immunohistochemistry-Paraffin: CYP4V2 Antibody (M29-P3B10) [NBP2-50214] -
Immunohistochemistry-Paraffin: CYP4V2 Antibody (M29-P3B10) [NBP2-50214] - Immunohistochemistry was performed on formalin-fixed, paraffin-embedded normal and primary colorectal tumour tissue sections using neat culture supernatants of anti-CYP4V2 (M29-P3B10). Photomicrographs show negative staining of anti-CYP4V2 [M29-P3B10] in normal colon mucosa and strong immunostaining in primary colorectal tumour. Cytoplasmic localisation of CYP4V2 is also observed.![Immunohistochemistry: CYP4V2 Antibody (M29-P3B10) - BSA Free [NBP2-50214]](https://resources.rndsystems.com/images/products/CYP4V2-Antibody-M29-P3B10-Immunohistochemistry-NBP2-50214-img0001.jpg "Immunohistochemistry: CYP4V2 Antibody (M29-P3B10) - BSA Free [NBP2-50214]")
Immunohistochemistry: CYP4V2 Antibody (M29-P3B10) - BSA Free [NBP2-50214]
Immunohistochemistry: CYP4V2 Antibody (M29-P3B10) [NBP2-50214] - CYP4V2 neat supernatant.Applications for CYP4V2 Antibody (M29-P3B10) - BSA Free
Application
Recommended Usage
ELISA
1:100 - 1:2000
Immunohistochemistry
1:10 - 1:500
Western Blot
1:100 - 1:2000
Application Notes
Positive control(s): ELISA- Peptide immunogen; Western Blot- Human liver microsomes; IHC- Human liver.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CYP4V2
Alternate Names
BCD, CYP4AH1, cytochrome P450 4V2, cytochrome P450, family 4, subfamily V, polypeptide 2, EC 1.14, EC 1.14.14.1, FLJ18432, MGC43534
Gene Symbol
CYP4V2
Additional CYP4V2 Products
Product Documents for CYP4V2 Antibody (M29-P3B10) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CYP4V2 Antibody (M29-P3B10) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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