CYP7A1 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-04836

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all CYP7A1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 390-504 of human CYP7A1 (NP_000771.2). YPQLMHLDPEIYPDPLTFKYDRYLDENGKTKTTFYCNGLKLKYYYMPFGSGATICPGRLFAIHEIKQFLILMLSYFELELIEGQAKCPPLDQSRAGLGILPPLNDIEFKYKFKHL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CYP7A1 Antibody - Azide and BSA Free

Western Blot: CYP7A1 AntibodyAzide and BSA Free [NBP3-04836]

Western Blot: CYP7A1 AntibodyAzide and BSA Free [NBP3-04836]

Western Blot: CYP7A1 Antibody [NBP3-04836] - Analysis of extracts of various cell lines, using CYP7A1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.
CYP7A1 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: CYP7A1 Antibody - Azide and BSA Free [NBP3-04836] -

Immunocytochemistry/ Immunofluorescence: CYP7A1 Antibody - Azide and BSA Free [NBP3-04836] - Immunofluorescence analysis of HepG2 cells using CYP7A1 Rabbit pAb (A10615) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for CYP7A1 Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CYP7A1

The CYP7A1 gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic r

Long Name

Cytochrome P450 7A1

Alternate Names

CP7A, CYP7, CYPVII

Gene Symbol

CYP7A1

Additional CYP7A1 Products

Product Documents for CYP7A1 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CYP7A1 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CYP7A1 Antibody - Azide and BSA Free

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Protocols

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FAQs

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