CYP8B1 Antibody (M15-P3B7) - BSA Free
Novus Biologicals | Catalog # NBP2-50218
Key Product Details
Species Reactivity
Human, Rat
Applications
Immunohistochemistry, Western Blot, ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # M15-P3B7
Format
BSA Free
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Product Specifications
Immunogen
Peptide sequence- QPSHDVRFR (amino acids 486 - 494)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for CYP8B1 Antibody (M15-P3B7) - BSA Free
Western Blot: CYP8B1 Antibody (M15-P3B7) [NBP2-50218]
![Immunohistochemistry: CYP8B1 Antibody (M15-P3B7) - BSA Free [NBP2-50218]](https://resources.rndsystems.com/images/products/CYP8B1-Antibody-M15-P3B7-Immunohistochemistry-NBP2-50218-img0004.jpg "Immunohistochemistry: CYP8B1 Antibody (M15-P3B7) - BSA Free [NBP2-50218]")
Immunohistochemistry: CYP8B1 Antibody (M15-P3B7) - BSA Free [NBP2-50218]
Immunohistochemistry: CYP8B1 Antibody (M15-P3B7) [NBP2-50218] - CYP8B1 normal colon mucosa![Immunohistochemistry: CYP8B1 Antibody (M15-P3B7) - BSA Free [NBP2-50218]](https://resources.rndsystems.com/images/products/CYP8B1-Antibody-M15-P3B7-Immunohistochemistry-NBP2-50218-img0001.jpg "Immunohistochemistry: CYP8B1 Antibody (M15-P3B7) - BSA Free [NBP2-50218]")
Immunohistochemistry: CYP8B1 Antibody (M15-P3B7) - BSA Free [NBP2-50218]
Immunohistochemistry: CYP8B1 Antibody (M15-P3B7) [NBP2-50218] - Staining of a human liver cross section.![Immunohistochemistry: CYP8B1 Antibody (M15-P3B7) - BSA Free [NBP2-50218]](https://resources.rndsystems.com/images/products/CYP8B1-Antibody-M15-P3B7-Immunohistochemistry-NBP2-50218-img0003.jpg "Immunohistochemistry: CYP8B1 Antibody (M15-P3B7) - BSA Free [NBP2-50218]")
Immunohistochemistry: CYP8B1 Antibody (M15-P3B7) - BSA Free [NBP2-50218]
Immunohistochemistry: CYP8B1 Antibody (M15-P3B7) [NBP2-50218] - Staining of a lymph node cross section from colorectal cancer tissue.![Immunohistochemistry: CYP8B1 Antibody (M15-P3B7) - BSA Free [NBP2-50218]](https://resources.rndsystems.com/images/products/CYP8B1-Antibody-M15-P3B7-Immunohistochemistry-NBP2-50218-img0005.jpg "Immunohistochemistry: CYP8B1 Antibody (M15-P3B7) - BSA Free [NBP2-50218]")
Immunohistochemistry: CYP8B1 Antibody (M15-P3B7) - BSA Free [NBP2-50218]
Immunohistochemistry: CYP8B1 Antibody (M15-P3B7) [NBP2-50218] - Staining of CYP8B1 in normal liver tissue.Applications for CYP8B1 Antibody (M15-P3B7) - BSA Free
Application
Recommended Usage
ELISA
1:100 - 1:2000
Immunohistochemistry
1:10 - 1:500
Western Blot
1:100 - 1:2000
Application Notes
Positive control(s): ELISA- Peptide immunogen; Western Blot- Human liver microsomes; IHC- Human liver.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CYP8B1
Alternate Names
CP8B, CYP127 alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase, CYPVIIIB1, Cytochrome P450 8B1, cytochrome P450, family 8, subfamily B, polypeptide 1, cytochrome P450, subfamily VIIIB (sterol 12-alpha-hydroxylase), polypeptide 1,7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase, EC 1.14.13.95, FLJ17826,7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase, Sterol 12-alpha-hydroxylase
Gene Symbol
CYP8B1
Additional CYP8B1 Products
Product Documents for CYP8B1 Antibody (M15-P3B7) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CYP8B1 Antibody (M15-P3B7) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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