Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25430

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2144 expressed in HEK293

Format

Azide and BSA Free
View all Cyr61/CCN1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human Cyr61/CCN1

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free

Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free

Western Blot: Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free [NBP3-25430] -

Western Blot: Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free [NBP3-25430] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CYR61 antibody [HL2144] (NBP3-25430) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free [NBP3-25430] -

Immunocytochemistry/Immunofluorescence: Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free [NBP3-25430] - CYR61 antibody [HL2144] detects CYR61 protein at secreted protein by immunofluorescent analysis. Sample: C2C12 cells were fixed in ice-cold MeOH for 5 min. Green: CYR61 stained by CYR61 antibody [HL2144] (NBP3-25430) diluted at 1:500. Blue: Fluoroshield with DAPI.
Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free

Western Blot: Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free [NBP3-25430] -

Western Blot: Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free [NBP3-25430] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with CYR61 antibody [HL2144] (NBP3-25430) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free

Immunohistochemistry-Paraffin: Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free [NBP3-25430] -

Immunohistochemistry-Paraffin: Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free [NBP3-25430] - CYR61 antibody [HL2144] detects CYR61 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. CYR61 stained by CYR61 antibody [HL2144] (NBP3-25430) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free

Western Blot: Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free [NBP3-25430] -

Western Blot: Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free [NBP3-25430] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with CYR61 antibody [HL2144] (NBP3-25430) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Applications for Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cyr61/CCN1

CYR61 (Cysteine-rich angiogenic inducer 61) is a 381 amino acid secreted protein. It promotes cell proliferation, chemotaxis, angiogenesis extracellular matrix formation and cell adhesion and this appears to make CYR61 play a role in wound healing in skin fibroblasts. CYR61 expression is induced by growth factors. It interacts with several integrins and with heparan sulfate proteoglycan. CYR61 may play a role in T-cell non-Hodgkin's disease, breast cancer, epithelial neoplasia, hepatocellular carcinoma, ischemia, gastric cancer, endometriosis, prostate carcinoma, prostatitis, prostate cancer, breast carcinoma, cardiomyopathy, melanoma and adenocarcinoma.

Long Name

Cysteine-Rich, Angiogenic Inducer 61

Alternate Names

CCN1, GIG1, IGFBP-10

Gene Symbol

CCN1

Additional Cyr61/CCN1 Products

Product Documents for Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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