Cytochrome b5 Outer Mitochondrial Membrane Antibody - BSA Free
Novus Biologicals | Catalog # NBP3-48074
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot, ELISA, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
cytochrome b5 type B(outer mitochondrial membrane). (Uniprot# O43169)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Cytochrome b5 Outer Mitochondrial Membrane Antibody - BSA Free
Immunohistochemistry: Cytochrome b5 Outer Mitochondrial Membrane Antibody - BSA Free [NBP3-48074] -
Immunohistochemistry: Cytochrome b5 Outer Mitochondrial Membrane Antibody - BSA Free [NBP3-48074] - Immunohistochemistry of paraffin-embedded human testis using (Cytochrome b5 Outer Mitochondrial Membrane antibody) at dilution of 1:100
Western Blot: Cytochrome b5 Outer Mitochondrial Membrane Antibody - BSA Free [NBP3-48074] -
Western Blot: Cytochrome b5 Outer Mitochondrial Membrane Antibody - BSA Free [NBP3-48074] - Mouse lung tissue were subjected to SDS PAGE followed by western blot with (Cytochrome b5 Outer Mitochondrial Membrane antibody) at dilution of 1:500
Immunoprecipitation: Cytochrome b5 Outer Mitochondrial Membrane Antibody - BSA Free [NBP3-48074] -
Immunoprecipitation: Cytochrome b5 Outer Mitochondrial Membrane Antibody - BSA Free [NBP3-48074] - IP Result of anti-Cytochrome b5 Outer Mitochondrial Membrane (IP:, 3ug; Detection: 1:500) with mouse lung tissue lysate 4000ug.Applications for Cytochrome b5 Outer Mitochondrial Membrane Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:20-1:200
Immunoprecipitation
1:200-1:1000
Western Blot
1:500-1:2000
Application Notes
Theoretical Molecular Weight is 20-21 kDa
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS, 50% Glycerol, pH (7.3)
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Cytochrome b5 Outer Mitochondrial Membrane
Alternate Names
CYB5M, CYB5-M, CYPB5M, Cytochrome b5 outer mitochondrial membrane isoform, cytochrome b5 type B, cytochrome b5 type B (outer mitochondrial membrane), DKFZp686M0619, OMB5, outer mitochondrial membrane cytochrome b5, type 2 cyt-b5
Gene Symbol
CYB5B
Additional Cytochrome b5 Outer Mitochondrial Membrane Products
Product Documents for Cytochrome b5 Outer Mitochondrial Membrane Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Cytochrome b5 Outer Mitochondrial Membrane Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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