Cytochrome c Antibody (7H8.2C12) - Azide and BSA Free

Flow Cytometry: Cytochrome c Antibody (7H8.2C12) - Azide and BSA Free [NBP2-80686]

Flow Cytometry: Cytochrome c Antibody (7H8.2C12) - Azide and BSA Free [NBP2-80686] - Using the Alexa Fluor 488 direct conjugate, an intracellular stain was performed on HeLa cells with Cytochrome c (7H8.2C12) antibody NB100-56503AF488 (blue) and a matched isotype control NBP2-27231AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 488.

Western Blot: Cytochrome c Antibody (7H8.2C12)Azide and BSA Free [NBP2-80686]

Western Blot: Cytochrome c Antibody (7H8.2C12) - Azide and BSA Free [NBP2-80686] - Analysis using the biotin conjugate of NB100-56503. Detection of Cytochrome C in 15 mg of HeLa cell lysate using NB100-55775 at 1:1000. A 15 kDa band is detected.

Flow Cytometry: Cytochrome c Antibody (7H8.2C12) - Azide and BSA Free [NBP2-80686]

Flow Cytometry: Cytochrome c Antibody (7H8.2C12) - Azide and BSA Free [NBP2-80686] - Detection of Cytochrome C in Human HeLa Cell Line by Flow Cytometry. Human HeLa cell line was stained with Mouse Anti- Cytochrome C Monoclonal Antibody (Catalog # NB100-56503, filled histogram), or Mouse IgG2B isotype control (Catalog # MAB0041, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a Bio-Techne brand. Image from the standard format of this antibody.

Simple Western: Cytochrome c Antibody (7H8.2C12)Azide and BSA Free [NBP2-80686]

Simple Western: Cytochrome c Antibody (7H8.2C12) - Azide and BSA Free [NBP2-80686] - Lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for Cytochrome c at approximately 23 kDa (as indicated) using 2.5 ug/mL of Mouse Anti-Cytochrome c Monoclonal Antibody (Catalog # NB100-56503). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Image from the standard format of this antibody.