Cytochrome c Antibody (7H8.2C12) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-80686
![Western Blot: Cytochrome c Antibody (7H8.2C12)Azide and BSA Free [NBP2-80686]](https://resources.rndsystems.com/images/products/Cytochrome-c-Antibody-7H8-2C12-Azide-and-BSA-Free-Western-Blot-NBP2-80686-img0002.jpg "Western Blot: Cytochrome c Antibody (7H8.2C12)Azide and BSA Free [NBP2-80686]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Human, Mouse, Rat, Drosophila, Rabbit
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoblotting, Flow Cytometry, Flow (Cell Surface), Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Simple Western, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 7H8.2C12
Format
Azide and BSA Free
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Product Specifications
Immunogen
Synthetic peptides corresponding to amino acids 1-80, 81-104 and 66-104 of pigeon CYT were used as the immunogen (Jemmerson et al. 1991). The antibody recognizes an epitope within amino acids 93-104 of pigeon cytochrome C based on competitive ELISA results (Jemmerson et al. 1991).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Scientific Data Images for Cytochrome c Antibody (7H8.2C12) - Azide and BSA Free
Western Blot: Cytochrome c Antibody (7H8.2C12)Azide and BSA Free [NBP2-80686]
Western Blot: Cytochrome c Antibody (7H8.2C12) - Azide and BSA Free [NBP2-80686] - Human HeLa lysate probed with Cytochrome C antibody at 0.1 ug/ml. Image from the standard format of this antibody.![Immunocytochemistry/ Immunofluorescence: Cytochrome c Antibody (7H8.2C12) - Azide and BSA Free [NBP2-80686]](https://resources.rndsystems.com/images/products/Cytochrome-c-Antibody-7H8-2C12-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80686-img0003.jpg "Immunocytochemistry/ Immunofluorescence: Cytochrome c Antibody (7H8.2C12) - Azide and BSA Free [NBP2-80686]")
Immunocytochemistry/ Immunofluorescence: Cytochrome c Antibody (7H8.2C12) - Azide and BSA Free [NBP2-80686]
Immunocytochemistry/Immunofluorescence: Cytochrome c Antibody (7H8.2C12) - Azide and BSA Free [NBP2-80686] - p73 was detected in immersion fixed Hela human cell line using NB100-56503 at 25 ug/ml for 3 hours at room temperature. Cells were stained using the NorthernLights(TM) 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Staining was observed in the cytoplasm and mitochondria. Image from the standard format of this antibody.![Immunohistochemistry: Cytochrome c Antibody (7H8.2C12) - Azide and BSA Free [NBP2-80686]](https://resources.rndsystems.com/images/products/Cytochrome-c-Antibody-7H8-2C12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80686-img0004.jpg "Immunohistochemistry: Cytochrome c Antibody (7H8.2C12) - Azide and BSA Free [NBP2-80686]")
Immunohistochemistry: Cytochrome c Antibody (7H8.2C12) - Azide and BSA Free [NBP2-80686]
Immunohistochemistry: Cytochrome c Antibody (7H8.2C12) - Azide and BSA Free [NBP2-80686] - Cytochrome C was detected in immersion fixed paraffin-embedded sections of human heart using anti-human mouse monoclonal antibody (Catalog # NB100-56503) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detec![Flow Cytometry: Cytochrome c Antibody (7H8.2C12) - Azide and BSA Free [NBP2-80686]](https://resources.rndsystems.com/images/products/Cytochrome-c-Antibody-7H8-2C12-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80686-img0006.jpg "Flow Cytometry: Cytochrome c Antibody (7H8.2C12) - Azide and BSA Free [NBP2-80686]")
Flow Cytometry: Cytochrome c Antibody (7H8.2C12) - Azide and BSA Free [NBP2-80686]
Flow Cytometry: Cytochrome c Antibody (7H8.2C12) - Azide and BSA Free [NBP2-80686] - Using the Alexa Fluor 488 direct conjugate, an intracellular stain was performed on HeLa cells with Cytochrome c (7H8.2C12) antibody NB100-56503AF488 (blue) and a matched isotype control NBP2-27231AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 488.![Western Blot: Cytochrome c Antibody (7H8.2C12)Azide and BSA Free [NBP2-80686]](https://resources.rndsystems.com/images/products/Cytochrome-c-Antibody-7H8-2C12-Azide-and-BSA-Free-Western-Blot-NBP2-80686-img0001.jpg "Western Blot: Cytochrome c Antibody (7H8.2C12)Azide and BSA Free [NBP2-80686]")
Western Blot: Cytochrome c Antibody (7H8.2C12)Azide and BSA Free [NBP2-80686]
Western Blot: Cytochrome c Antibody (7H8.2C12) - Azide and BSA Free [NBP2-80686] - Analysis using the biotin conjugate of NB100-56503. Detection of Cytochrome C in 15 mg of HeLa cell lysate using NB100-55775 at 1:1000. A 15 kDa band is detected.![Flow Cytometry: Cytochrome c Antibody (7H8.2C12) - Azide and BSA Free [NBP2-80686]](https://resources.rndsystems.com/images/products/Cytochrome-c-Antibody-7H8-2C12-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80686-img0005.jpg "Flow Cytometry: Cytochrome c Antibody (7H8.2C12) - Azide and BSA Free [NBP2-80686]")
Flow Cytometry: Cytochrome c Antibody (7H8.2C12) - Azide and BSA Free [NBP2-80686]
Flow Cytometry: Cytochrome c Antibody (7H8.2C12) - Azide and BSA Free [NBP2-80686] - Detection of Cytochrome C in Human HeLa Cell Line by Flow Cytometry. Human HeLa cell line was stained with Mouse Anti- Cytochrome C Monoclonal Antibody (Catalog # NB100-56503, filled histogram), or Mouse IgG2B isotype control (Catalog # MAB0041, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a Bio-Techne brand. Image from the standard format of this antibody.![Simple Western: Cytochrome c Antibody (7H8.2C12)Azide and BSA Free [NBP2-80686]](https://resources.rndsystems.com/images/products/Cytochrome-c-Antibody-7H8-2C12-Azide-and-BSA-Free-Simple-Western-NBP2-80686-img0007.jpg "Simple Western: Cytochrome c Antibody (7H8.2C12)Azide and BSA Free [NBP2-80686]")
Simple Western: Cytochrome c Antibody (7H8.2C12)Azide and BSA Free [NBP2-80686]
Simple Western: Cytochrome c Antibody (7H8.2C12) - Azide and BSA Free [NBP2-80686] - Lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for Cytochrome c at approximately 23 kDa (as indicated) using 2.5 ug/mL of Mouse Anti-Cytochrome c Monoclonal Antibody (Catalog # NB100-56503). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Image from the standard format of this antibody.Applications for Cytochrome c Antibody (7H8.2C12) - Azide and BSA Free
Application
Recommended Usage
Flow (Cell Surface)
reported in scientific literature (PMID 16467206)
Flow (Intracellular)
reported in scientific literature (Mohr et al (2004))
Flow Cytometry
1:20-1:2000
Immunoblotting
reported in scientific literature (PMID 16436379)
Immunocytochemistry/ Immunofluorescence
1:10-1:500. Use reported in scientific literature (Yamasaki et al (2006))
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500. Use reported in scientific literature (Fujimara et al (1998))
Simple Western
2.5 ug/ml
Western Blot
0.05-0.5 ug/ml
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Cytochrome c
Alternate Names
CYCS
Gene Symbol
CYCS
Additional Cytochrome c Products
Product Documents for Cytochrome c Antibody (7H8.2C12) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Cytochrome c Antibody (7H8.2C12) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
