Cytochrome P450 2B6 Antibody (OTI3D5)

Novus Biologicals | Catalog # NBP2-01800

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Monkey

Cited:

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI3D5
View all Cytochrome P450 2B6 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human CYP2B6(NP_000758) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

56.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cytochrome P450 2B6 Antibody (OTI3D5)

Western Blot: Cytochrome P450 2B6 Antibody (OTI3D5) [NBP2-01800]

Western Blot: Cytochrome P450 2B6 Antibody (OTI3D5) [NBP2-01800]

Western Blot: Cytochrome P450 2B6 Antibody (3D5) [NBP2-01800] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Cytochrome P450 2B6 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunocytochemistry/ Immunofluorescence: Cytochrome P450 2B6 Antibody (OTI3D5) [NBP2-01800]

Immunocytochemistry/ Immunofluorescence: Cytochrome P450 2B6 Antibody (OTI3D5) [NBP2-01800]

Immunocytochemistry/Immunofluorescence: Cytochrome P450 2B6 Antibody (3D5) [NBP2-01800] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cytochrome P450 2B6.
Immunohistochemistry-Paraffin: Cytochrome P450 2B6 Antibody (OTI3D5) [NBP2-01800]

Immunohistochemistry-Paraffin: Cytochrome P450 2B6 Antibody (OTI3D5) [NBP2-01800]

Immunohistochemistry-Paraffin: Cytochrome P450 2B6 Antibody (3D5) [NBP2-01800] - Staining of paraffin-embedded Human liver tissue using anti-Cytochrome P450 2B6 mouse monoclonal antibody.
Flow Cytometry: Cytochrome P450 2B6 Antibody (OTI3D5) [NBP2-01800]

Flow Cytometry: Cytochrome P450 2B6 Antibody (OTI3D5) [NBP2-01800]

Flow Cytometry: Cytochrome P450 2B6 Antibody (3D5) [NBP2-01800] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Cytochrome P450 2B6 antibody, and then analyzed by flow cytometry.
Western Blot: Cytochrome P450 2B6 Antibody (OTI3D5) [NBP2-01800]

Western Blot: Cytochrome P450 2B6 Antibody (OTI3D5) [NBP2-01800]

Western Blot: Cytochrome P450 2B6 Antibody (3D5) [NBP2-01800] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytochrome P450 2B6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cytochrome P450 2B6.
Immunohistochemistry-Paraffin: Cytochrome P450 2B6 Antibody (OTI3D5) [NBP2-01800]

Immunohistochemistry-Paraffin: Cytochrome P450 2B6 Antibody (OTI3D5) [NBP2-01800]

Immunohistochemistry-Paraffin: Cytochrome P450 2B6 Antibody (3D5) [NBP2-01800] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-Cytochrome P450 2B6 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Cytochrome P450 2B6 Antibody (OTI3D5) [NBP2-01800]

Immunohistochemistry-Paraffin: Cytochrome P450 2B6 Antibody (OTI3D5) [NBP2-01800]

Immunohistochemistry-Paraffin: Cytochrome P450 2B6 Antibody (3D5) [NBP2-01800] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Cytochrome P450 2B6 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Cytochrome P450 2B6 Antibody (OTI3D5) [NBP2-01800]

Immunohistochemistry-Paraffin: Cytochrome P450 2B6 Antibody (OTI3D5) [NBP2-01800]

Immunohistochemistry-Paraffin: Cytochrome P450 2B6 Antibody (3D5) [NBP2-01800] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Cytochrome P450 2B6 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Cytochrome P450 2B6 Antibody (OTI3D5) [NBP2-01800]

Immunohistochemistry-Paraffin: Cytochrome P450 2B6 Antibody (OTI3D5) [NBP2-01800]

Immunohistochemistry-Paraffin: Cytochrome P450 2B6 Antibody (3D5) [NBP2-01800] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-Cytochrome P450 2B6 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Cytochrome P450 2B6 Antibody (OTI3D5) [NBP2-01800]

Immunohistochemistry-Paraffin: Cytochrome P450 2B6 Antibody (OTI3D5) [NBP2-01800]

Immunohistochemistry-Paraffin: Cytochrome P450 2B6 Antibody (3D5) [NBP2-01800] - Staining of paraffin-embedded Human colon tissue using anti-Cytochrome P450 2B6 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Cytochrome P450 2B6 Antibody (OTI3D5) [NBP2-01800]

Immunohistochemistry-Paraffin: Cytochrome P450 2B6 Antibody (OTI3D5) [NBP2-01800]

Immunohistochemistry-Paraffin: Cytochrome P450 2B6 Antibody (3D5) [NBP2-01800] - Staining of paraffin-embedded Human Kidney tissue using anti-Cytochrome P450 2B6 mouse monoclonal antibody.
Flow Cytometry: Cytochrome P450 2B6 Antibody (OTI3D5) [NBP2-01800]

Flow Cytometry: Cytochrome P450 2B6 Antibody (OTI3D5) [NBP2-01800]

Flow Cytometry: Cytochrome P450 2B6 Antibody (3D5) [NBP2-01800] - Analysis of Jurkat cells, using anti-Cytochrome P450 2B6 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Applications for Cytochrome P450 2B6 Antibody (OTI3D5)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cytochrome P450 2B6

This gene, CYP2B6, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to metabolize some xenobiotics, such as the anti-cancer drugs cyclophosphamide and ifosphamide. Transcript variants for this gene have been described; however, it has not been resolved whether these transcripts are in fact produced by this gene or by a closely related pseudogene, CYP2B7. Both the gene and the pseudogene are located in the middle of a CYP2A pseudogene found in a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q. [provided by RefSeq].

Long Name

Cytochrome P450 2B6

Alternate Names

CYP2B6, CYPIIB6, EC 1.14.13.-

Entrez Gene IDs

1555 (Human)

Gene Symbol

CYP2B6

Additional Cytochrome P450 2B6 Products

Product Documents for Cytochrome P450 2B6 Antibody (OTI3D5)

Certificate of Analysis

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Product Specific Notices for Cytochrome P450 2B6 Antibody (OTI3D5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Cytochrome P450 2B6 Antibody (OTI3D5)

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Protocols

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