Cytoglobin Antibody

Novus Biologicals | Catalog # NBP2-16084

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Predicted:

Bovine (95%), Rhesus Macaque (97%), Sheep (96%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytoglobin. The exact sequence is proprietary.

Reactivity Notes

Dog (82%).

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cytoglobin Antibody

Western Blot: Cytoglobin Antibody [NBP2-16084]

Western Blot: Cytoglobin Antibody [NBP2-16084]

Western Blot: Cytoglobin Antibody [NBP2-16084] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Cytoglobin antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Cytoglobin Antibody [NBP2-16084]

Immunocytochemistry/ Immunofluorescence: Cytoglobin Antibody [NBP2-16084]

Immunocytochemistry/Immunofluorescence: Cytoglobin Antibody [NBP2-16084] - Immunofluorescence analysis of methanol-fixed A431, using antibody at 1:500 dilution.
Western Blot: Cytoglobin Antibody [NBP2-16084]

Western Blot: Cytoglobin Antibody [NBP2-16084]

Western Blot: Cytoglobin Antibody [NBP2-16084] - Sample (30 ug of whole cell lysate) A: IMR32 12% SDS PAGE gel, diluted at 1:2000.
Western Blot: Cytoglobin Antibody [NBP2-16084]

Western Blot: Cytoglobin Antibody [NBP2-16084]

Western Blot: Cytoglobin Antibody [NBP2-16084] - Sample (50 ug of whole cell lysate) A: Mouse Heart, 12% SDS PAGE gel, diluted at 1:1000.

Applications for Cytoglobin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry-Paraffin

Reported in scientific literature (PMID: 26835715)

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Cytoglobin

Cytoglobin is a ubiquitously expressed hexacoordinate hemoglobin that may facilitate diffusion of oxygen through tissues, scavenge nitric oxide or other reactive oxygen species, or serve a protective function during oxidative stress (Trent and Hargrove, 2002 [PubMed 11893755]).[supplied by OMIM]

Alternate Names

cytoglobin, HGBHistoglobin, histoglobin, STAPHGb, Stellate cell activation-associated protein

Gene Symbol

CYGB

UniProt

Additional Cytoglobin Products

Product Documents for Cytoglobin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Cytoglobin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for Cytoglobin Antibody

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

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