Cytokeratin 1 Antibody (3C10F7)

Novus Biologicals | Catalog # NB110-89450

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Porcine, Bovine, Canine, Equine, Feline, Goat

Cited:

Porcine, Bovine, Canine, Equine, Feline, Goat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry Free-Floating, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3C10F7
View all Cytokeratin 1 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of Cytokeratin 1 expressed in E. Coli.

Reactivity Notes

Porcine, Bovine, Goat, Equine, Canine, and Feline reactivities reported in scientific literature (PMID: 26190143).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Cytokeratin 1 Antibody (3C10F7)

Western Blot: Cytokeratin 1 Antibody (3C10F7)BSA Free [NB110-89450]

Western Blot: Cytokeratin 1 Antibody (3C10F7)BSA Free [NB110-89450]

Western Blot: Cytokeratin 1 Antibody (3C10F7) [NB110-89450] - Analysis using CK1 mouse mAb against truncated CK1 recombinant protein.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 1 Antibody (3C10F7) - BSA Free [NB110-89450]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 1 Antibody (3C10F7) - BSA Free [NB110-89450]

Immunocytochemistry/Immunofluorescence: Cytokeratin 1 Antibody (3C10F7) [NB110-89450] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Cytokeratin 1 (3C10F7) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (3C10F7) - BSA Free [NB110-89450]

Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (3C10F7) - BSA Free [NB110-89450]

Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (3C10F7) [NB110-89450] - Analysis of human liver tissue (A), colon carcinoma (B), lung carcinoma (C) and esophagus tissue (D), showing membrane localization using CK1 mouse mAb with DAB staining.

Applications for Cytokeratin 1 Antibody (3C10F7)

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

2-5 ug/ml

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500 - 1:2000
Application Notes
Use in Immunohistochemistry free floating reported in scientific literature (PMID: 26190143).

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cytokeratin 1

CK1 (also designated Cytokeratin 1 or KRT1 ),with 644-amino acid protein (about 70kDa), is a member of the keratin family. Cytokeratins play a critical role in differentiation and tissue specialization, and they function to maintain the overall structural integrity of epithelial cells.CK1 consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. CK1 is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma.

Alternate Names

CK1, CK-1, cytokeratin 1, Cytokeratin-1, cytokeratin-1,67 kDa cytokeratin, EHK, EHK1, epidermolytic hyperkeratosis 1, EPPK, Hair alpha protein, K1, keratin 1, keratin, type II cytoskeletal 1, Keratin-1, KRT1A, KRTA, NEPPK, Type-II keratin Kb1

Entrez Gene IDs

3848 (Human)

Gene Symbol

KRT1

Additional Cytokeratin 1 Products

Product Documents for Cytokeratin 1 Antibody (3C10F7)

Certificate of Analysis

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Product Specific Notices for Cytokeratin 1 Antibody (3C10F7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Cytokeratin 1 Antibody (3C10F7)

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Protocols

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