Cytokeratin 1 Antibody (LHK1) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80688

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # LHK1

Format

Azide and BSA Free
View all Cytokeratin 1 Primary Antibodies »

Product Specifications

Immunogen

C-terminal peptide sequence of human Cytokeratin 1 [UniProt# P04264]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cytokeratin 1 Antibody (LHK1) - Azide and BSA Free

Western Blot: Cytokeratin 1 Antibody (LHK1)Azide and BSA Free [NBP2-80688]

Western Blot: Cytokeratin 1 Antibody (LHK1)Azide and BSA Free [NBP2-80688]

Western Blot: Cytokeratin 1 Antibody (LHK1) - Azide and BSA Free [NBP2-80688] - Analysis of Cytokeratin 1 in Caco-2. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 1 Antibody (LHK1) - Azide and BSA Free [NBP2-80688]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 1 Antibody (LHK1) - Azide and BSA Free [NBP2-80688]

Immunocytochemistry/Immunofluorescence: Cytokeratin 1 Antibody (LHK1) - Azide and BSA Free [NBP2-80688] - Cytokeratin 1 antibody (LHK1) was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image from the standard format of this antibody.
Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (LHK1) - Azide and BSA Free [NBP2-80688]

Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (LHK1) - Azide and BSA Free [NBP2-80688]

Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (LHK1) - Azide and BSA Free [NBP2-80688] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human breast cancer using 5ug/ml conc. of Cytokeratin 1 antibody (clone LHK1) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) usi
Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (LHK1) - Azide and BSA Free [NBP2-80688]

Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (LHK1) - Azide and BSA Free [NBP2-80688]

Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (LHK1) - Azide and BSA Free [NBP2-80688] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human breast cancer using 5ug/ml conc. of Cytokeratin 1 antibody (clone LHK1) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) usi

Applications for Cytokeratin 1 Antibody (LHK1) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

5 ug/ml

Immunohistochemistry

2-10 ug/ml

Immunohistochemistry-Frozen

5-10 ug/ml

Immunohistochemistry-Paraffin

2-5 ug/ml

Western Blot

0.5-2 ug/ml
Application Notes
This Cytokeratin 1 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-frozen sections, Immunohistochemistry-paraffin sections and Western blot.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cytokeratin 1

Cytokeratin 1 is a heterotetramer of two type I as well as two type II keratins localized in the cell membrane and is generally associated with keratin-10 (KRT10). It has been suggested to regulate the activity of kinases such as PKC/SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein kinase C (RACK1/GNB2L1). Cytokeratin 1 and its heterodimer partner KRT10 are the major constituents of 4 intermediate filament cytoskeleton in suprabasal epidermis/keratinocytes, and their upregulation precedes expression of filaggrin and cornified envelope proteins. Covalent crosslinking of cytokeratin 1 but not of KRT10 to these proteins, in addition to a complex series of lipids, ultimately forms the cornified/lipid envelope, that constitutes the epidermal barrier together with Langerhans cells. Defects in cytokeratin 1 have been linked to epidermolytic hyperkeratosis (EHK), ichthyosis hystrix Curth-Macklin type (IHCM), palmoplantar keratoderma non-epidermolytic (NEPPK), ichthyosis annular epidermolytic (AEI), palmoplantar keratoderma striate type 3 (SPPK3).

Alternate Names

CK1, CK-1, cytokeratin 1, Cytokeratin-1, cytokeratin-1,67 kDa cytokeratin, EHK, EHK1, epidermolytic hyperkeratosis 1, EPPK, Hair alpha protein, K1, keratin 1, keratin, type II cytoskeletal 1, Keratin-1, KRT1A, KRTA, NEPPK, Type-II keratin Kb1

Gene Symbol

KRT1

Additional Cytokeratin 1 Products

Product Documents for Cytokeratin 1 Antibody (LHK1) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Cytokeratin 1 Antibody (LHK1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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