Cytokeratin 18 Antibody (DA7)

Novus Biologicals | Catalog # NBP2-29409

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Bovine (Negative), Canine (Negative), Hamster (Negative), Mouse (Negative), Porcine (Negative), Rat (Negative), Sheep (Negative)

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # DA7
View all Cytokeratin 18 Primary Antibodies »

Product Specifications

Immunogen

Human breast cancer PMC 42 cells

Reactivity Notes

Does not react with Mouse, Rat, Sheep, Hamster, Bovine, Canine or Porcine.

Localization

Cytoplasmic

Marker

Epithelial Marker

Specificity

This monoclonal antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33176)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Cytokeratin 18 Antibody (DA7)

Western Blot: Cytokeratin 18 Antibody (DA7) [NBP2-29409]

Western Blot: Cytokeratin 18 Antibody (DA7) [NBP2-29409]

Western Blot: Cytokeratin 18 Antibody (DA7) [NBP2-29409] - Western Blot Analysis of HCT116 cell lysate using Cytokeratin 18 Antibody (DA7).
Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody (DA7) [NBP2-29409]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody (DA7) [NBP2-29409]

Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (DA7) [NBP2-29409] - Confocal immunofluorescence image of MCF cells using Cytokeratin 18 Antibody (DA7). Green (CF488) and Red Dot is used to label the nuclei.
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (DA7) [NBP2-29409]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (DA7) [NBP2-29409]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (DA7) [NBP2-29409] - Formalin-paraffin human colon cancer stained with Cytokeratin 18 Ab (DA7). Note cytoplasmic staining of tumor cells.
Western Blot: Cytokeratin 18 Antibody (DA7) [NBP2-29409]

Western Blot: Cytokeratin 18 Antibody (DA7) [NBP2-29409]

Western Blot: Cytokeratin 18 Antibody (DA7) [NBP2-29409] - Analysis of Cytokeratin 18 in HeLa lysate using Cytokeratin 18 (CK18) antibody at 1 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Simple Western: Cytokeratin 18 Antibody (DA7) [NBP2-29409]

Simple Western: Cytokeratin 18 Antibody (DA7) [NBP2-29409]

Simple Western: Cytokeratin 18 Antibody (DA7) [NBP2-29409] - Simple Western lane view shows a specific band for Cytokeratin 18 in 0.2 mg/ml of HeLa lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Simple Western: Cytokeratin 18 Antibody (DA7) [NBP2-29409]

Simple Western: Cytokeratin 18 Antibody (DA7) [NBP2-29409]

Simple Western: Cytokeratin 18 Antibody (DA7) [NBP2-29409] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 18 antibody was used at 10 ug/ml dilution of HeLa lysates(s) respectively.

Applications for Cytokeratin 18 Antibody (DA7)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Simple Western

10 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
In Simple Western only 10 - 15 ul of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate, separated by Size, antibody dilution of 10 ug/mL, apparent MW was 56 kDa.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cytokeratin 18

Keratins, also called cytokeratins, comprise a family of filamentous structural proteins that form the intermediate filaments of epithelial cells. Cytokeratin proteins are differentially expressed depending on epithelial cell type and degree of differentiation. Antibody to a given cytokeratin can be useful as a marker in of itself or as part of an antibody panel to help identify or classify tissue origin. The DA7 antibody clone reacts with Keratin 18 (CK18), a 45 kDa molecular weight polypeptide that is expressed in combination with Keratin 8. Cytokeratin 18 and 8 are the most commonly expressed intermediate filaments in single layer or simple epithelial tissues.

Alternate Names

CK-18, CYK18, Keratin 18, KRT18

Gene Symbol

KRT18

UniProt

P05783 (Human)

Additional Cytokeratin 18 Products

Product Documents for Cytokeratin 18 Antibody (DA7)

Certificate of Analysis

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Product Specific Notices for Cytokeratin 18 Antibody (DA7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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