Cytokeratins are cytoskeletal intermediate filament proteins that are classified as type I (acidic) and type II (neutral to basic) and associate into heterotetramers.
Cytokeratin 18 Antibody (DA7) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-33176
Key Product Details
Species Reactivity
Human, Bovine (Negative), Canine (Negative), Hamster (Negative), Mouse (Negative), Porcine (Negative), Rat (Negative), Sheep (Negative)
Applications
Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # DA7
Format
Azide and BSA Free
Loading...
Product Specifications
Immunogen
Human breast cancer PMC 42 cells
Reactivity Notes
Does not react with Mouse, Rat, Sheep, Hamster, Bovine, Canine or Porcine.
Localization
Cytoplasmic
Marker
Epithelial Marker
Specificity
This monoclonal antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-29409).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Cytokeratin 18 Antibody (DA7) - Azide and BSA Free
![Western Blot: Cytokeratin 18 Antibody (DA7)Azide and BSA Free [NBP2-33176]](https://resources.rndsystems.com/images/products/Cytokeratin-18-Antibody-DA7-Azide-and-BSA-Free-Western-Blot-NBP2-33176-img0005.jpg "Western Blot: Cytokeratin 18 Antibody (DA7)Azide and BSA Free [NBP2-33176]")
Western Blot: Cytokeratin 18 Antibody (DA7)Azide and BSA Free [NBP2-33176]
Western Blot: Cytokeratin 18 Antibody (DA7) - Azide and BSA Free [NBP2-33176] - Analysis of Cytokeratin 18 in HeLa lysate using Cytokeratin 18 (CK18) antibody at 1 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.![Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody (DA7) - Azide and BSA Free [NBP2-33176]](https://resources.rndsystems.com/images/products/Cytokeratin-18-Antibody-DA7-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-33176-img0003.jpg "Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody (DA7) - Azide and BSA Free [NBP2-33176]")
Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody (DA7) - Azide and BSA Free [NBP2-33176]
Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (DA7) - Azide and BSA Free [NBP2-33176] - Confocal immunofluorescence image of MCF cells using Cytokeratin 18 Antibody (DA7). Green (CF488) and Red Dot is used to label the nuclei.![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (DA7) - Azide and BSA Free [NBP2-33176]](https://resources.rndsystems.com/images/products/Cytokeratin-18-Antibody-DA7-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-33176-img0007.jpg "Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (DA7) - Azide and BSA Free [NBP2-33176]")
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (DA7) - Azide and BSA Free [NBP2-33176]
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (DA7) - Azide and BSA Free [NBP2-33176] - Formalin-paraffin human colon cancer stained with Cytokeratin 18 Ab (DA7). Note cytoplasmic staining of tumor cells.![Western Blot: Cytokeratin 18 Antibody (DA7)Azide and BSA Free [NBP2-33176]](https://resources.rndsystems.com/images/products/Cytokeratin-18-Antibody-DA7-Azide-and-BSA-Free-Western-Blot-NBP2-33176-img0004.jpg "Western Blot: Cytokeratin 18 Antibody (DA7)Azide and BSA Free [NBP2-33176]")
Western Blot: Cytokeratin 18 Antibody (DA7)Azide and BSA Free [NBP2-33176]
Western Blot: Cytokeratin 18 Antibody (DA7) - Azide and BSA Free [NBP2-33176] - Western Blot Analysis of HCT116 cell lysate using Cytokeratin 18 Antibody (DA7).![Simple Western: Cytokeratin 18 Antibody (DA7)Azide and BSA Free [NBP2-33176]](https://resources.rndsystems.com/images/products/Cytokeratin-18-Antibody-DA7-Azide-and-BSA-Free-Simple-Western-NBP2-33176-img0001.jpg "Simple Western: Cytokeratin 18 Antibody (DA7)Azide and BSA Free [NBP2-33176]")
Simple Western: Cytokeratin 18 Antibody (DA7)Azide and BSA Free [NBP2-33176]
Simple Western: Cytokeratin 18 Antibody (DA7) - Azide and BSA Free [NBP2-33176] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 18 antibody was used at 10 ug/ml dilution of HeLa lysates(s) respectively.![Simple Western: Cytokeratin 18 Antibody (DA7)Azide and BSA Free [NBP2-33176]](https://resources.rndsystems.com/images/products/Cytokeratin-18-Antibody-DA7-Azide-and-BSA-Free-Simple-Western-NBP2-33176-img0002.jpg "Simple Western: Cytokeratin 18 Antibody (DA7)Azide and BSA Free [NBP2-33176]")
Simple Western: Cytokeratin 18 Antibody (DA7)Azide and BSA Free [NBP2-33176]
Simple Western: Cytokeratin 18 Antibody (DA7) - Azide and BSA Free [NBP2-33176] - Simple Western lane view shows a specific band for Cytokeratin 18 in 0.2 mg/ml of HeLa lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.Applications for Cytokeratin 18 Antibody (DA7) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Simple Western
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
See Simple Western Antibody Database for Simple Western validation
Optimal dilution for a specific application should be determined.
See Simple Western Antibody Database for Simple Western validation
Flow Cytometry Panel Builder
Bio-Techne Knows Flow Cytometry
Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Cytokeratin 18
Alternate Names
CK-18, CYK18, Keratin 18, KRT18
Gene Symbol
KRT18
Additional Cytokeratin 18 Products
Product Documents for Cytokeratin 18 Antibody (DA7) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Cytokeratin 18 Antibody (DA7) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Customer Reviews for Cytokeratin 18 Antibody (DA7) - Azide and BSA Free
There are currently no reviews for this product. Be the first to review Cytokeratin 18 Antibody (DA7) - Azide and BSA Free and earn rewards!
Have you used Cytokeratin 18 Antibody (DA7) - Azide and BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...