Cytokeratin 18 Antibody (OTI1E1)

Novus Biologicals | Catalog # NBP1-47817

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1E1
View all Cytokeratin 18 Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 69-372 of human CK18 (NP_000215) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Marker

Epithelial Marker

Specificity

This antibody is specific for Homo sapiens keratin 18 (KRT18), transcript variant 2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

47.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cytokeratin 18 Antibody (OTI1E1)

Western Blot: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817]

Western Blot: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817]

Western Blot: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Analysis of extracts (35ug) from 9 different cell lines by using anti-KRT18 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817]

Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY KRT18.
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Staining of paraffin-embedded Human pancreas tissue using anti-KRT18 mouse monoclonal antibody.
Western Blot: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817]

Western Blot: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817]

Western Blot: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Extracts (10ug) from 10 Human tissue by using Cytokeratin 18 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
Western Blot: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817]

Western Blot: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817]

Western Blot: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Cytokeratin 18 antibody ( 1E1) at 1:1000 dilution with HepG2 cell lysate
Western Blot: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817]

Western Blot: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817]

Western Blot: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT18 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT18.
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Staining of paraffin-embedded Human colon tissue within the normal limits. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Staining of paraffin-embedded Human liver tissue within the normal limits. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120C for 3 min, (1:6000).
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Staining of paraffin-embedded Human pancreas tissue within the normal limits. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-KRT18 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-KRT18 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-KRT18 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-KRT18 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Staining of paraffin-embedded Human breast tissue using anti-KRT18 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Staining of paraffin-embedded Human colon tissue using anti-KRT18 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Staining of paraffin-embedded Human endometrium tissue using anti-KRT18 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Staining of paraffin-embedded Human Kidney tissue using anti-KRT18 mouse monoclonal antibody.

Applications for Cytokeratin 18 Antibody (OTI1E1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:500-1000

Reviewed Applications

Read 1 review rated 5 using NBP1-47817 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cytokeratin 18

Keratin 18 together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.

Alternate Names

CK-18, CYK18, Keratin 18, KRT18

Entrez Gene IDs

3875 (Human)

Gene Symbol

KRT18

Additional Cytokeratin 18 Products

Product Documents for Cytokeratin 18 Antibody (OTI1E1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Cytokeratin 18 Antibody (OTI1E1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Cytokeratin 18 Antibody (OTI1E1) (1)

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  • Cytokeratin 18 Antibody (OTI1E1)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: PANC-1 human pancreatic carcinoma cell line
    Species: Human
    Verified Customer | Posted 10/09/2023
    Panc1 cell lysate treated with control or drugs (A-D) were performed WB. 10 ug each protein wrer loaded. size markers are 25, 20, 15 kDa, respectively.
    Cytokeratin 18 Antibody (OTI1E1) NBP1-47817

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