Cytokeratins are cytoskeletal intermediate filament proteins that are classified as type I (acidic) and type II (neutral to basic) and associate into heterotetramers.
Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-71086
![Western Blot: Cytokeratin 18 Antibody (OTI1E1)Azide and BSA Free [NBP2-71086]](https://resources.rndsystems.com/images/products/Cytokeratin-18-Antibody-OTI1E1-Azide-and-BSA-Free-Western-Blot-NBP2-71086-img0004.jpg "Western Blot: Cytokeratin 18 Antibody (OTI1E1)Azide and BSA Free [NBP2-71086]")
Key Product Details
Species Reactivity
Human, Monkey
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI1E1
Format
Azide and BSA Free
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Product Specifications
Immunogen
Human recombinant protein fragment corresponding to amino acids 69-372 of human CK18 (NP_000215) produced in E.coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
47.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free
Western Blot: Cytokeratin 18 Antibody (OTI1E1)Azide and BSA Free [NBP2-71086]
Western Blot: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT18 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT18.![Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086]](https://resources.rndsystems.com/images/products/Cytokeratin-18-Antibody-OTI1E1-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-71086-img0005.jpg "Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086]")
Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086]
Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY KRT18.![Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086]](https://resources.rndsystems.com/images/products/Cytokeratin-18-Antibody-OTI1E1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71086-img0017.jpg "Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086]")
Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086]
Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086] - Staining of paraffin-embedded Human liver tissue within the normal limits. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120C for 3 min, (1:6000).![Flow Cytometry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086]](https://resources.rndsystems.com/images/products/Cytokeratin-18-Antibody-OTI1E1-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71086-img0011.jpg "Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086]")
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086]
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086] - Staining of paraffin-embedded Human colon tissue using anti-KRT18 mouse monoclonal antibody.![Western Blot: Cytokeratin 18 Antibody (OTI1E1)Azide and BSA Free [NBP2-71086]](https://resources.rndsystems.com/images/products/Cytokeratin-18-Antibody-OTI1E1-Azide-and-BSA-Free-Western-Blot-NBP2-71086-img0001.jpg "Western Blot: Cytokeratin 18 Antibody (OTI1E1)Azide and BSA Free [NBP2-71086]")
Western Blot: Cytokeratin 18 Antibody (OTI1E1)Azide and BSA Free [NBP2-71086]
Western Blot: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086] - Analysis of extracts (35ug) from 9 different cell lines by using anti-KRT18 monoclonal antibody.![Western Blot: Cytokeratin 18 Antibody (OTI1E1)Azide and BSA Free [NBP2-71086]](https://resources.rndsystems.com/images/products/Cytokeratin-18-Antibody-OTI1E1-Azide-and-BSA-Free-Western-Blot-NBP2-71086-img0002.jpg "Western Blot: Cytokeratin 18 Antibody (OTI1E1)Azide and BSA Free [NBP2-71086]")
Western Blot: Cytokeratin 18 Antibody (OTI1E1)Azide and BSA Free [NBP2-71086]
Western Blot: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086] - Cytokeratin 18 antibody ( 1E1) at 1:1000 dilution with HepG2 cell lysate![Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086]](https://resources.rndsystems.com/images/products/Cytokeratin-18-Antibody-OTI1E1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71086-img0006.jpg "Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086]")
Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086]
Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-KRT18 mouse monoclonal antibody.![Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086]](https://resources.rndsystems.com/images/products/Cytokeratin-18-Antibody-OTI1E1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71086-img0007.jpg "Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086]")
Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086]
Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-KRT18 mouse monoclonal antibody.![Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086]](https://resources.rndsystems.com/images/products/Cytokeratin-18-Antibody-OTI1E1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71086-img0008.jpg "Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086]")
Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086]
Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-KRT18 mouse monoclonal antibody.![Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086]](https://resources.rndsystems.com/images/products/Cytokeratin-18-Antibody-OTI1E1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71086-img0009.jpg "Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086]")
Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086]
Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-KRT18 mouse monoclonal antibody.![Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086]](https://resources.rndsystems.com/images/products/Cytokeratin-18-Antibody-OTI1E1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71086-img0010.jpg "Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086]")
Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086]
Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086] - Staining of paraffin-embedded Human breast tissue using anti-KRT18 mouse monoclonal antibody.![Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086]](https://resources.rndsystems.com/images/products/Cytokeratin-18-Antibody-OTI1E1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71086-img0012.jpg "Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086]")
Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086]
Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086] - Staining of paraffin-embedded Human endometrium tissue using anti-KRT18 mouse monoclonal antibody.![Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086]](https://resources.rndsystems.com/images/products/Cytokeratin-18-Antibody-OTI1E1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71086-img0013.jpg "Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086]")
Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086]
Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086] - Staining of paraffin-embedded Human Kidney tissue using anti-KRT18 mouse monoclonal antibody.![Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086]](https://resources.rndsystems.com/images/products/Cytokeratin-18-Antibody-OTI1E1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71086-img0014.jpg "Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086]")
Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086]
Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086] - Staining of paraffin-embedded Human pancreas tissue using anti-KRT18 mouse monoclonal antibody.![Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086]](https://resources.rndsystems.com/images/products/Cytokeratin-18-Antibody-OTI1E1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71086-img0015.jpg "Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086]")
Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086]
Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.![Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086]](https://resources.rndsystems.com/images/products/Cytokeratin-18-Antibody-OTI1E1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71086-img0016.jpg "Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086]")
Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086]
Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086] - Staining of paraffin-embedded Human colon tissue within the normal limits. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.![Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086]](https://resources.rndsystems.com/images/products/Cytokeratin-18-Antibody-OTI1E1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71086-img0018.jpg "Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086]")
Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086]
Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086] - Staining of paraffin-embedded Human pancreas tissue within the normal limits. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.Applications for Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:50
Western Blot
1:500-1000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3)
Format
Azide and BSA Free
Preservative
8% Trehalose
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Calculators
Background: Cytokeratin 18
Alternate Names
CK-18, CYK18, Keratin 18, KRT18
Gene Symbol
KRT18
Additional Cytokeratin 18 Products
Product Documents for Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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