Cytokeratin 19 Antibody (SA30-06)

Western Blot: Cytokeratin 19 Antibody (SA30-06) [NBP3-32248]

Western Blot: Cytokeratin 19 Antibody (SA30-06) [NBP3-32248] - All lanes: Western blot analysis of Cytokeratin 19 with anti-Cytokeratin 19 antibody [SA30-06] (NBP3-32248) at 1:5,000 dilution.
Lane 1: Wild-type HepG2 whole cell lysate (20 ug).
Lane 2/3: Cytokeratin 19 knockout HepG2 whole cell lysate (20 ug).

NBP3-32248 was shown to specifically react with Cytokeratin 19 in wild-type HepG2 cells. No band was observed when Cytokeratin 19 knockout samples were tested. Wild-type and Cytokeratin 19 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-Cytokeratin 19 Antibody (ET1601-6, 1/5,000) and anti-HSP90 antibody (ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Cytokeratin 19 Antibody (SA30-06) [NBP3-32248]

Immunohistochemistry: Cytokeratin 19 Antibody (SA30-06) [NBP3-32248] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Cytokeratin 19 antibody (NBP3-32248) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32248) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry: Cytokeratin 19 Antibody (SA30-06) [NBP3-32248]

Immunohistochemistry: Cytokeratin 19 Antibody (SA30-06) [NBP3-32248] - Immunofluorescence analysis of paraffin-embedded human kidney tissue labeling Cytokeratin 19 (NBP3-32248).

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 19 (NBP3-32248, red) at 1/500 dilution at +4℃ overnight, washed with PBS.

Goat Anti-Rabbit IgG H&L (iFluor™ 594) was used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).