Cytokeratin 19 Antibody (SPM561) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-34426
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM561
Format
Azide and BSA Free
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Product Specifications
Immunogen
Human mammary epithelial organoids. (Uniprot: P08727)
Localization
Cytoplasmic
Marker
Pancreatic Stem Cell Marker
Specificity
This Ab reacts with the rod domain of human cytokeratin 19 (CK19), a polypeptide of 40kDa. CK19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50%-60% of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-34379).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Cytokeratin 19 Antibody (SPM561) - Azide and BSA Free
![Western Blot: Cytokeratin 19 Antibody (SPM561)Azide and BSA Free [NBP2-34426]](https://resources.rndsystems.com/images/products/Cytokeratin-19-Antibody-SPM561-Azide-and-BSA-Free-Western-Blot-NBP2-34426-img0005.jpg "Western Blot: Cytokeratin 19 Antibody (SPM561)Azide and BSA Free [NBP2-34426]")
Western Blot: Cytokeratin 19 Antibody (SPM561)Azide and BSA Free [NBP2-34426]
Western Blot: Cytokeratin 19 Antibody (SPM561) - Azide and BSA Free [NBP2-34426] - Western Blot Analysis of HepG2 cell lysate using Cytokeratin 19 Antibody (SPM561).![Immunocytochemistry/ Immunofluorescence: Cytokeratin 19 Antibody (SPM561) - Azide and BSA Free [NBP2-34426]](https://resources.rndsystems.com/images/products/Cytokeratin-19-Antibody-SPM561-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-34426-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Cytokeratin 19 Antibody (SPM561) - Azide and BSA Free [NBP2-34426]")
Immunocytochemistry/ Immunofluorescence: Cytokeratin 19 Antibody (SPM561) - Azide and BSA Free [NBP2-34426]
Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody (SPM561) - Azide and BSA Free [NBP2-34426] - Immunofluorescence Analysis of MeOH-fixed MCF-7 cells labeling CK19 using Cytokeratin 19 Antibody (SPM561) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (SPM561) - Azide and BSA Free [NBP2-34426]](https://resources.rndsystems.com/images/products/Cytokeratin-19-Antibody-SPM561-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34426-img0006.jpg "Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (SPM561) - Azide and BSA Free [NBP2-34426]")
Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (SPM561) - Azide and BSA Free [NBP2-34426]
Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (SPM561) - Azide and BSA Free [NBP2-34426] - Formalin-fixed, paraffin-embedded human Bladder carcinoma stained with cytokeratin 19 Monoclonal Antibody (SPM561).![Flow Cytometry: Cytokeratin 19 Antibody (SPM561) - Azide and BSA Free [NBP2-34426]](https://resources.rndsystems.com/images/products/Cytokeratin-19-Antibody-SPM561-Azide-and-BSA-Free-Flow-Cytometry-NBP2-34426-img0002.jpg "Flow Cytometry: Cytokeratin 19 Antibody (SPM561) - Azide and BSA Free [NBP2-34426]")
Flow Cytometry: Cytokeratin 19 Antibody (SPM561) - Azide and BSA Free [NBP2-34426]
Flow Cytometry: Cytokeratin 19 Antibody (SPM561) - Azide and BSA Free [NBP2-34426] - Flow Cytometric Analysis of MeOH-fixed MCF-7 cells using Cytokeratin 19 Antibody (SPM561) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).Applications for Cytokeratin 19 Antibody (SPM561) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5-1ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2ug/ml
Immunohistochemistry-Paraffin
0.5-1ug/ml
Western Blot
0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Cytokeratin 19
Alternate Names
CK19, EndoC, K19, Krt19
Gene Symbol
KRT19
Additional Cytokeratin 19 Products
Product Documents for Cytokeratin 19 Antibody (SPM561) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Cytokeratin 19 Antibody (SPM561) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
