Cytokeratin 5 Antibody (2C2) - BSA Free

Novus Biologicals | Catalog # NBP2-22194

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Key Product Details

Species Reactivity

Human, Mouse, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2C2

Format

BSA Free
View all Cytokeratin 5 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Cytokeratin 5 expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Localization

Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

62.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cytokeratin 5 Antibody (2C2) - BSA Free

Western Blot: Cytokeratin 5 Antibody (2C2)BSA Free [NBP2-22194]

Western Blot: Cytokeratin 5 Antibody (2C2)BSA Free [NBP2-22194]

Western Blot: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Analysis using Cytokeratin 5 mouse mAb against A431 (1), MCF-7 (2), HeLa (3), HepG2 (4), 3T3-L1 (5), and COS-7 (6) cell lysate.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 5 Antibody (2C2) - BSA Free [NBP2-22194]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 5 Antibody (2C2) - BSA Free [NBP2-22194]

Immunocytochemistry/Immunofluorescence: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Immunofluorescence analysis of HeLa cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (2C2) - BSA Free [NBP2-22194]

Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (2C2) - BSA Free [NBP2-22194]

Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Analysis of stomach cancer tissues using Cytokeratin 5 mouse mAb with DAB staining.
Flow Cytometry: Cytokeratin 5 Antibody (2C2) - BSA Free [NBP2-22194]

Flow Cytometry: Cytokeratin 5 Antibody (2C2) - BSA Free [NBP2-22194]

Flow Cytometry: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - An intracellular stain was performed on HeLa cells with Cytokeratin 5 Antibody (2C2) NBP2-22194APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to allophycocyanin.
Western Blot: Cytokeratin 5 Antibody (2C2)BSA Free [NBP2-22194]

Western Blot: Cytokeratin 5 Antibody (2C2)BSA Free [NBP2-22194]

Western Blot: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Analysis using Cytokeratin 5 mAb against human Cytokeratin 5 (AA: 158-272) recombinant protein. (Expected MW is 33.3 kDa)
Immunocytochemistry/ Immunofluorescence: Cytokeratin 5 Antibody (2C2) - BSA Free [NBP2-22194]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 5 Antibody (2C2) - BSA Free [NBP2-22194]

Immunocytochemistry/Immunofluorescence: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Immunofluorescence analysis of HeLa cells using Cytokeratin 5 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (2C2) - BSA Free [NBP2-22194]

Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (2C2) - BSA Free [NBP2-22194]

Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Analysis of breast cancer tissues using Cytokeratin 5 mouse mAb with DAB staining.
Flow Cytometry: Cytokeratin 5 Antibody (2C2) - BSA Free [NBP2-22194]

Flow Cytometry: Cytokeratin 5 Antibody (2C2) - BSA Free [NBP2-22194]

Flow Cytometry: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Analysis of HeLa cells using Cytokeratin 5 mouse mAb (green) and negative control (red).
ELISA: Cytokeratin 5 Antibody (2C2) - BSA Free [NBP2-22194]

ELISA: Cytokeratin 5 Antibody (2C2) - BSA Free [NBP2-22194]

ELISA: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)

Applications for Cytokeratin 5 Antibody (2C2) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.

Reviewed Applications

Read 1 review rated 1 using NBP2-22194 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cytokeratin 5

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.

Alternate Names

CK-5, DDD, EBS2, Keratin 5, KRT5

Gene Symbol

KRT5

Additional Cytokeratin 5 Products

Product Documents for Cytokeratin 5 Antibody (2C2) - BSA Free

Certificate of Analysis

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Product Specific Notices for Cytokeratin 5 Antibody (2C2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Cytokeratin 5 Antibody (2C2) - BSA Free

Customer Reviews for Cytokeratin 5 Antibody (2C2) - BSA Free (1)

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  • Cytokeratin 5 Antibody (2C2)
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Human keratinocytes
    Species: Human
    Verified Customer | Posted 12/27/2019
    : The antibody showed intense unspecific stain at the stratum corneum. Only at 40x magnification can see slightly more staining at the basal and suprabasal cells.
    Cytokeratin 5 Antibody (2C2) - BSA Free NBP2-22194
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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