Cytokeratin 5 Antibody (3P3T9)

Novus Biologicals | Catalog # NBP3-15374

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3P3T9 expressed in HEK293
View all Cytokeratin 5 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 491-590 of human Cytokeratin 5 (KRT5) (P13647). ISVVTSSVSSGYGSGSGYGGGLGGGLGGGLGGGLAGGSSGSYYSSSSGGVGLGGGLSVGGSGFSASSGRGLGVGFGSGGGSSSSVKFVSTTSSSRKSFKS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cytokeratin 5 Antibody (3P3T9)

Western Blot: Cytokeratin 5 Antibody (3P3T9) [NBP3-15374]

Western Blot: Cytokeratin 5 Antibody (3P3T9) [NBP3-15374]

Western Blot: Cytokeratin 5 Antibody (3P3T9) [NBP3-15374] - Western blot analysis of extracts of A-431 cells, using Cytokeratin 5 (KRT5) Rabbit mAb (NBP3-15374) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Western Blot: Cytokeratin 5 Antibody (3P3T9) [NBP3-15374]

Western Blot: Cytokeratin 5 Antibody (3P3T9) [NBP3-15374]

Western Blot: Cytokeratin 5 Antibody (3P3T9) [NBP3-15374] - Western blot analysis of extracts of various cell lines, using Cytokeratin 5 (KRT5) Rabbit mAb (NBP3-15374) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Cytokeratin 5 Antibody (3P3T9)

Immunocytochemistry/ Immunofluorescence: Cytokeratin 5 Antibody (3P3T9) [Cytokeratin 5] -

Immunocytochemistry/ Immunofluorescence: Cytokeratin 5 Antibody (3P3T9) [Cytokeratin 5] - Confocal imaging of paraffin-embedded Rat skin using Cytokeratin 5 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.
Cytokeratin 5 Antibody (3P3T9)

Immunohistochemistry: Cytokeratin 5 Antibody (3P3T9) [Cytokeratin 5] -

Immunohistochemistry: Cytokeratin 5 Antibody (3P3T9) [Cytokeratin 5] - Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using Cytokeratin 5 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Cytokeratin 5 Antibody (3P3T9)

Immunohistochemistry: Cytokeratin 5 Antibody (3P3T9) [Cytokeratin 5] -

Immunohistochemistry: Cytokeratin 5 Antibody (3P3T9) [Cytokeratin 5] - Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using Cytokeratin 5 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Applications for Cytokeratin 5 Antibody (3P3T9)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunocytochemistry/ Immunofluorescence

1:200 - 1:400

Immunohistochemistry

1:2000 - 1:8000

Immunohistochemistry-Paraffin

1:2000 - 1:8000

Western Blot

1:1000 - 1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cytokeratin 5

Keratins are a family of structurally related proteins that form the intermediate filament cytoskeleton in epithelial cells. The 58-kD keratin CK-5 is highly similar to other type II keratins and less similar to type I keratins and other intermediate filament proteins. The 58-kD keratin is regulated by retinoids in several tissues and is one of four keratins abundantly expressed in epidermal keratinocytes, where it may be important in maintaining structural integrity of the integument (1). Keratin 5 (CK-5) mRNA and protein are shown to be expressed in normal mammary epithelial cells in culture and are absent from tumor-derived cell lines. This makes CK-5 an important marker in the tumorigenic process, distinguishing normal from tumor cells, and decreased CK-5 expression correlates with tumorigenic progression (2). Dowling-Degos disease (DDD) is an autosomal dominant genodermatosis characterized by progressive and disfiguring reticulate hyperpigmentation of the flexures. Loss of function of CK-5 suggests a crucial role for keratins in the organization of cell adhesion, melanosome uptake, organelle transport, and nuclear anchorage (3).

Alternate Names

CK-5, DDD, EBS2, Keratin 5, KRT5

Gene Symbol

KRT5

Additional Cytokeratin 5 Products

Product Documents for Cytokeratin 5 Antibody (3P3T9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Cytokeratin 5 Antibody (3P3T9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Cytokeratin 5 Antibody (3P3T9)

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Protocols

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FAQs

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