Cytokeratin 5 Antibody (CL9297) - BSA Free

Novus Biologicals | Catalog # NBP2-88923

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL9297

Format

BSA Free
View all Cytokeratin 5 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a synthetic peptide derived from P13647, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Cytokeratin 5 Antibody (CL9297) - BSA Free

Western Blot: Cytokeratin 5 Antibody (CL9297) [NBP2-88923]

Western Blot: Cytokeratin 5 Antibody (CL9297) [NBP2-88923]

Western Blot: Cytokeratin 5 Antibody (CL9297) [NBP2-88923] - Analysis in human skin tissue.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 5 Antibody (CL9297) [NBP2-88923]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 5 Antibody (CL9297) [NBP2-88923]

Immunocytochemistry/Immunofluorescence: Cytokeratin 5 Antibody (CL9297) [NBP2-88923] - Staining of A-431 cells using the Anti-KRT5 monoclonal antibody, showing specific staining in intermediate filaments in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (CL9297) [NBP2-88923]

Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (CL9297) [NBP2-88923]

Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (CL9297) [NBP2-88923] - Analysis in human prostate and kidney tissues using NBP2-88923 antibody. Corresponding KRT5 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (CL9297) [NBP2-88923]

Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (CL9297) [NBP2-88923]

Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (CL9297) [NBP2-88923] - Staining of human cervix shows moderate to strong cytoplasmic positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (CL9297) [NBP2-88923]

Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (CL9297) [NBP2-88923]

Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (CL9297) [NBP2-88923] - Staining of human kidney shows no positivity in cells in tubules as expected.
Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (CL9297) [NBP2-88923]

Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (CL9297) [NBP2-88923]

Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (CL9297) [NBP2-88923] - Staining of human lung cancer, squamous cell carcinoma shows strong cytoplasmic positivity.
Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (CL9297) [NBP2-88923]

Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (CL9297) [NBP2-88923]

Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (CL9297) [NBP2-88923] - Staining of human prostate shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (CL9297) [NBP2-88923]

Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (CL9297) [NBP2-88923]

Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (CL9297) [NBP2-88923] - Staining of human skin shows strong cytoplasmic positivity in squamous epithelial cells.
Cytokeratin 5 Antibody (CL9297) - BSA Free

Cytokeratin 5 (CL9297) in A431 Human Cell Line.

Cytokeratin 5 (CL9297) was detected in immersion fixed A431 human skin carcinoma cell line using Mouse anti-Cytokeratin 5 (CL9297) Protein A Purified Monoclonal Antibody conjugated to DyLight 550 (Catalog # NBP2-88923) (red) at 2 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Applications for Cytokeratin 5 Antibody (CL9297) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cytokeratin 5

Keratins are a family of structurally related proteins that form the intermediate filament cytoskeleton in epithelial cells. The 58-kD keratin CK-5 is highly similar to other type II keratins and less similar to type I keratins and other intermediate filament proteins. The 58-kD keratin is regulated by retinoids in several tissues and is one of four keratins abundantly expressed in epidermal keratinocytes, where it may be important in maintaining structural integrity of the integument (1). Keratin 5 (CK-5) mRNA and protein are shown to be expressed in normal mammary epithelial cells in culture and are absent from tumor-derived cell lines. This makes CK-5 an important marker in the tumorigenic process, distinguishing normal from tumor cells, and decreased CK-5 expression correlates with tumorigenic progression (2). Dowling-Degos disease (DDD) is an autosomal dominant genodermatosis characterized by progressive and disfiguring reticulate hyperpigmentation of the flexures. Loss of function of CK-5 suggests a crucial role for keratins in the organization of cell adhesion, melanosome uptake, organelle transport, and nuclear anchorage (3).

Alternate Names

CK-5, DDD, EBS2, Keratin 5, KRT5

Gene Symbol

KRT5

Additional Cytokeratin 5 Products

Product Documents for Cytokeratin 5 Antibody (CL9297) - BSA Free

Certificate of Analysis

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Product Specific Notices for Cytokeratin 5 Antibody (CL9297) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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