Cytokeratin 71 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-17040

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Sheep

Cited:

Mouse

Predicted:

Bovine (91%), Canine (91%), Feline (93%), Rat (91%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytokeratin 71. The exact sequence is proprietary.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25705371).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cytokeratin 71 Antibody - BSA Free

Western Blot: Cytokeratin 71 Antibody [NBP2-17040]

Western Blot: Cytokeratin 71 Antibody [NBP2-17040]

Western Blot: Cytokeratin 71 Antibody [NBP2-17040] - Cytokeratin 71 Sample (30 ug of whole cell lysate) A: HepG2 7. 5% SDS PAGE gel, diluted at 1:5000.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 71 Antibody [NBP2-17040]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 71 Antibody [NBP2-17040]

Cytokeratin-71-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-17040-img0008.jpg
Immunohistochemistry-Paraffin: Cytokeratin 71 Antibody [NBP2-17040]

Immunohistochemistry-Paraffin: Cytokeratin 71 Antibody [NBP2-17040]

Immunohistochemistry-Paraffin: Cytokeratin 71 Antibody [NBP2-17040] - Colon carcinoma. KRT71 antibody [N3C3] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 71 Antibody [NBP2-17040]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 71 Antibody [NBP2-17040]

Cytokeratin-71-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-17040-img0006.jpg
Immunocytochemistry/ Immunofluorescence: Cytokeratin 71 Antibody [NBP2-17040]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 71 Antibody [NBP2-17040]

Cytokeratin-71-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-17040-img0007.jpg
Cytokeratin 71 Antibody

Immunocytochemistry/ Immunofluorescence: Cytokeratin 71 Antibody [NBP2-17040] -

Immunocytochemistry/ Immunofluorescence: Cytokeratin 71 Antibody [NBP2-17040] - Densities of proliferating & apoptotic cells in a spine follicle. Longitudinal sections of a 28-day-old Acomys.(A) pH3 (green), K71 (red), & Hoechst (blue) immunostaining. (B) BrdU (green), K71 (red), & Hoechst (blue) immunostaining. (C) BrdU (green), TUNEL (red), & Hoechst (blue) immunostaining. (D–F) Color-coded local relative densities in a radius that corresponds to 3% of the image diagonal (blue: low density, red: high density) of cells labelled with Hoechst (D), BrdU (E), & TUNEL (F). Scale bars: 100 μm. Image collected & cropped by CiteAb from the following publication (https://evodevojournal.biomedcentral.com/articles/10.1186/2041-9139-5-33), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Cytokeratin 71 Antibody

Immunocytochemistry/ Immunofluorescence: Cytokeratin 71 Antibody [NBP2-17040] -

Immunocytochemistry/ Immunofluorescence: Cytokeratin 71 Antibody [NBP2-17040] - Observed & simulated cell proliferation & cell death along a spine follicle. Transverse sections (28-day-old Acomys) are taken along the spine long axis, from the basis of the follicle (rows A-C), to the DP-medulla transition (rows D-E), to the collapse of the keratinized medulla (rows F-G). H&E, Hematoxylin & Eosin staining. Immunostaining: Kpan, Pan-Keratin (red); Lmn, Laminin (green); BrdU, 5-bromo-2'-deoxyuridine (green); K71, keratin 71 (red); pH3, phospho-Histone H3 (green); TUNEL (red), Hoechst (blue). Arrows: IRS, DP: dermal papilla, Mx: matrix, C: cortex, M: medulla. Simulated transverse sections (Sim) are snapshots of the Supplementary Movie 2 showing time evolution of a simulated follicle. Scale bars: 50 μm. Image collected & cropped by CiteAb from the following publication (https://evodevojournal.biomedcentral.com/articles/10.1186/2041-9139-5-33), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Cytokeratin 71 Antibody

Immunocytochemistry/ Immunofluorescence: Cytokeratin 71 Antibody [NBP2-17040] -

Immunocytochemistry/ Immunofluorescence: Cytokeratin 71 Antibody [NBP2-17040] - Observed & simulated cell proliferation & cell death along a spine follicle. Transverse sections (28-day-old Acomys) are taken along the spine long axis, from the basis of the follicle (rows A-C), to the DP-medulla transition (rows D-E), to the collapse of the keratinized medulla (rows F-G). H&E, Hematoxylin & Eosin staining. Immunostaining: Kpan, Pan-Keratin (red); Lmn, Laminin (green); BrdU, 5-bromo-2'-deoxyuridine (green); K71, keratin 71 (red); pH3, phospho-Histone H3 (green); TUNEL (red), Hoechst (blue). Arrows: IRS, DP: dermal papilla, Mx: matrix, C: cortex, M: medulla. Simulated transverse sections (Sim) are snapshots of the Supplementary Movie 2 showing time evolution of a simulated follicle. Scale bars: 50 μm. Image collected & cropped by CiteAb from the following publication (https://evodevojournal.biomedcentral.com/articles/10.1186/2041-9139-5-33), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Cytokeratin 71 Antibody

Immunocytochemistry/ Immunofluorescence: Cytokeratin 71 Antibody [NBP2-17040] -

Immunocytochemistry/ Immunofluorescence: Cytokeratin 71 Antibody [NBP2-17040] - Observed & simulated cell proliferation & cell death along a spine follicle. Transverse sections (28-day-old Acomys) are taken along the spine long axis, from the basis of the follicle (rows A-C), to the DP-medulla transition (rows D-E), to the collapse of the keratinized medulla (rows F-G). H&E, Hematoxylin & Eosin staining. Immunostaining: Kpan, Pan-Keratin (red); Lmn, Laminin (green); BrdU, 5-bromo-2'-deoxyuridine (green); K71, keratin 71 (red); pH3, phospho-Histone H3 (green); TUNEL (red), Hoechst (blue). Arrows: IRS, DP: dermal papilla, Mx: matrix, C: cortex, M: medulla. Simulated transverse sections (Sim) are snapshots of the Supplementary Movie 2 showing time evolution of a simulated follicle. Scale bars: 50 μm. Image collected & cropped by CiteAb from the following publication (https://evodevojournal.biomedcentral.com/articles/10.1186/2041-9139-5-33), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Cytokeratin 71 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Cytokeratin 71 Antibody [NBP2-17040] -

KRT71 antibody [N3C3] detects KRT71 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: KRT71 protein stained by KRT71 antibody [N3C3] (NBP2-17040) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Applications for Cytokeratin 71 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Cytokeratin 71

Cytokeratin 71 belongs to a family of type II keratins that are specifically expressed in the inner root sheath of hair follicles.[supplied by OMIM]

Alternate Names

CK-71, Cytokeratin-71, hK6irs, hK6irs1, K6IRS1MGC119390, K71, KB34, Keratin 6 irs, keratin 71, keratin, type II cytoskeletal 71, Keratin-71, KRT6IRS, KRT6IRS1MGC119391, Type II inner root sheath-specific keratin-K6irs1, Type-II keratin Kb34

Gene Symbol

KRT71

UniProt

Additional Cytokeratin 71 Products

Product Documents for Cytokeratin 71 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Cytokeratin 71 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for Cytokeratin 71 Antibody - BSA Free

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Protocols

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