Cytokeratin, pan Antibody (PCK-26)

Novus Biologicals | Catalog # NB120-6401

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken, Equine, Feline, Fish, Goat, Guinea Pig, Hamster, Rabbit, Reptile, Sheep

Cited:

Human, Mouse, Rat, Feline, Ovine, Reptile

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # PCK-26
View all Cytokeratin, pan Primary Antibodies »

Product Specifications

Immunogen

This Cytokeratin, pan Antibody (PCK-26) was developed against the full length native protein (purified) (Human epidermis).

Reactivity Notes

Use in Equine reported in scientific literature (PMID:34372610) Cross reacts with: carp, lizard, snake. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Cytoplasmic

Marker

Epithelial marker

Specificity

PCK-26 recognizes the 58 kDa cytokeratin 5, the 56 kDa cytokeratin 6 and the 52 kDa cytokeratin 8 band in immunoblotting (Lane EB et al). The antibody recognizes an epitope located on the Type II cytokeratins 1, 5, 6, and 8. PCK-26 is a broad spectrum antibody which reacts specifically with a variety of normal, reactive, and neoplastic epithelial tissues. The antibody reacts with simple, cornifying, and non-cornifying squamous epithelia and pseudostratified epithelia.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Cytokeratin, pan Antibody (PCK-26)

Western Blot: Cytokeratin, pan Antibody (PCK-26) [NB120-6401]

Western Blot: Cytokeratin, pan Antibody (PCK-26) [NB120-6401]

Western Blot: Cytokeratin, pan Antibody (PCK-26) [NB120-6401] - Whole extract of HeLa cells was separated on SDS-PAGE and probed with Monoclonal Anti-Cytokeratin, pan Clone: PCK-26. WB was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lane 1: Antibody dilution 1:300. Lane 2: Antibody dilution 1:500. Lane 3: Antibody dilution 1:1000.
Immunocytochemistry/ Immunofluorescence: Cytokeratin, pan Antibody (PCK-26) [NB120-6401]

Immunocytochemistry/ Immunofluorescence: Cytokeratin, pan Antibody (PCK-26) [NB120-6401]

Immunocytochemistry/Immunofluorescence: Cytokeratin, pan Antibody (PCK-26) [NB120-6401] - Human pancreas cancer cells. Antibody at 1:250 dilution. Overnight incubation. No permeabilization. ICC/IF image submitted by a verified customer review.
Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (PCK-26) [NB120-6401]

Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (PCK-26) [NB120-6401]

Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (PCK-26) [NB120-6401] - Staining of FFPE human placenta sections with 1:300 Monoclonal Anti-Cytokeratin, pan Clone: PCK-26, followed by Goat Anti Mouse IgG (Fab)-FITC.
Immunohistochemistry: Cytokeratin, pan Antibody (PCK-26) [NB120-6401]

Immunohistochemistry: Cytokeratin, pan Antibody (PCK-26) [NB120-6401]

Cytokeratin-pan-Antibody-PCK-26-Immunohistochemistry-NB120-6401-img0014.jpg
Immunohistochemistry: Cytokeratin, pan Antibody (PCK-26) [NB120-6401]

Immunohistochemistry: Cytokeratin, pan Antibody (PCK-26) [NB120-6401]

Cytokeratin-pan-Antibody-PCK-26-Immunohistochemistry-NB120-6401-img0013.jpg
Cytokeratin, pan Antibody (PCK-26)

Immunocytochemistry/ Immunofluorescence: Cytokeratin, pan Antibody (PCK-26) [NB120-6401] -

Immunocytochemistry/ Immunofluorescence: Cytokeratin, pan Antibody (PCK-26) [NB120-6401] - Detection of EMT & increased motility in NHRI-HN1 cells. (A) Summary of the most enriched pathways associated with tumorigenesis in syngeneic mice by comparing tumorigenic NHRI-HN1 cells with nontumorigenic cells, including M1-2, M2-3 & NHRI-HN2, using GSEA analysis. Blue indicates a negative normalized enrichment score (NES) & orange indicates a positive NES. (B) Morphology of M1-2 & NHRI-HN1 cells via phase-contrast microscopy at 100× magnification. (C) Relative adhesion activity in M1-2 & NHRI-HN1 cells, determined by normalizing the mean OD 490 nm value of NHRI-HN1 cells to that of M1-2 cells. (D) Cells stained with Alexa Fluor 488 phalloidin, anti-pan-CK & anti-EGFR antibodies at 400× magnification. (E) Immunoblot analysis of epithelial (E-cadherin, alpha -catenin & beta -catenin), mesenchymal (N-cadherin & Vimentin) proteins & EMT-related transcription factors, including Twist, Snail & Slug in M1-2 & NHRI-HN1 cells. Protein levels were normalized to an internal control, alpha -tubulin. Ratios were determined by dividing the normalized protein levels in NHRI-HN1 cells by that in M1-2 cells. (F) Representative images (left) & relative data (right) for migration activity of M1-2 & NHRI-HN1 cells. (G) Representative images (left) & relative data (right) for invasion activity of M1-2 & NHRI-HN1 cells at 200× magnification. The relative migration or invasion activity was determined by normalizing the mean number of cells that have migrated or invaded per field of NHRI-HN1 cells to that of M1-2 cells. Error bars represent SE; ** p < 0.01; *** p < 0.001. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31878324), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Cytokeratin, pan Antibody (PCK-26)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:300

Immunohistochemistry-Frozen

1:300 - 1:600

Immunohistochemistry-Paraffin

1:300 - 1:600

Western Blot

1:100 - 1:2000

Reviewed Applications

Read 2 reviews rated 5 using NB120-6401 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.09% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cytokeratin, pan

Cytokeratins are a family of intermediate filamentous proteins that are expressed by epithelial cells (1,2). Cytokeratins range in size with a theoretic molecular weight varying from approximately 40 kDa to 68 kDa (2,3). The cytokeratin family consists of 20 polypeptides that are further divided into two main groups based on isoelectric point and molecular weight (1-3). The type I group are smaller, acidic polypeptides designated as cytokeratin 9 through cytokeratin 20 (CK9 - CK20) (1-4). Conversely, CK1 - CK8 belong to the type II group, classified as larger, basic or neutral polypeptides (1-4). Structurally, cytokeratins have homologous basic structure with other intermediate filaments; they possess a 300-315 amino acid (aa) central helical region that consists of four conserved domains (1A, 2A, 1B, and 2B) which are separated by linker domains (L1, L12, and L2) (1,5). Additionally, flanking this central region, both the amino- and carboxyl-terminal ends have a homologous subdomain (H), a variable domain (V), and charged end subdomains (E) (1). Furthermore, the central rod of one cytokeratin monomer binds with another monomer to form a coiled-coil dimer which subsequently binds another dimer to form a tertramer (3). Finally, many tetramers join together to ultimately form an intermediate filament of approximately 10nm in diameter (1-3, 5). Cytokeratins are expressed as pairs, typically with a type I and type II member; for example, CK10 pairs with CK1 (1,3).

Epithelial cells express multiple subtypes of cytokeratins which can be used to classify epithelial cell type or differentiation status, as well tumor progression or diagnosis (2). Cytokeratins are important for both stability and integrity of epithelial cells and function in intracellular signaling, from wound healing to apoptosis (1). Cytokeratins are useful immunohistochemistry tumor markers and antibodies to cytokeratins are a common pathological tool (1,3,6). Cytokeratin pan antibody is an antibody cocktail mixture that can detect multiple cytokeratins and reacts to multiple epithelial tissues (1,3,6). For example, AE-1/AE-3 is a commonly used specific pan cytokeratin that detects cytokeratins 1-8, 10, 14-16 and 19 (1,3,6).

Given the role of cytokeratins in the structural integrity of epithelial cells, mutations in cytokeratins have been shown to play a role in a variety of human diseases including epidermolysis bullosa simplex (EBS) (4,5). EBS is an autosomal dominant disorder that is caused by missense mutations in either CK5 or CK14 (5). Other known cytokeratin-related disorders include bullous ichthyosis, a skin disorder characterized by redness, blistering, and hyperkeratosis, and epidermolytic palmoplantar keratoderma (EPPK), which results in hyperkeratosis on the palms and soles of the body (7).

References

1. Awasthi, P., Thahriani, A., Bhattacharya, A., Awasthi, P., & Keratins, B. A. (2016). Keratins or cytokeratins: a review article. Journal of Advanced Medical and Dental Sciences Research. https://10.21276/jamdsr.2016.4.4.30

2. Southgate, J., Harnden, P., & Trejdosiewicz, L. K. (1999). Cytokeratin expression patterns in normal and malignant urothelium: a review of the biological and diagnostic implications. Histology and histopathology. https://doi.org/10.14670/HH-14.657

3. Belaldavar, C., Mane, D. R., Hallikerimath, S., Kale, A. D. (2016). Cytokeratins: Its role and expression profile in oral health and disease. Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology. https://doi.org/10.1016/j.ajoms.2015.08.001.

4. Linder S. (2007). Cytokeratin markers come of age. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. https://doi.org/10.1159/000107582

5. Jacob, J. T., Coulombe, P. A., Kwan, R., & Omary, M. B. (2018). Types I and II Keratin Intermediate Filaments. Cold Spring Harbor perspectives in biology. https://doi.org/10.1101/cshperspect.a018275

6. Ordonez N. G. (2013). Broad-spectrum immunohistochemical epithelial markers: a review. Human pathology. https://doi.org/10.1016/j.humpath.2012.11.016

7. McLean, W. H., & Moore, C. B. (2011). Keratin disorders: from gene to therapy. Human molecular genetics. https://doi.org/10.1093/hmg/ddr379

Alternate Names

AEI2, CK1, EHK, EPPK, K1, KRT1A, NEPPK

Entrez Gene IDs

3848 (Human); 300250 (Rat)

Gene Symbol

KRT1

UniProt

P04264 (Human)

Additional Cytokeratin, pan Products

Product Documents for Cytokeratin, pan Antibody (PCK-26)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Cytokeratin, pan Antibody (PCK-26)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Cytokeratin, pan Antibody (PCK-26)

Customer Reviews for Cytokeratin, pan Antibody (PCK-26) (2)

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Showing  1 - 2 of 2 reviews Showing All
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  • Cytokeratin, pan Antibody (PCK-26)
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: human pancreas cancer cells
    Species: Human
    Verified Customer | Posted 08/09/2019
    Dilution 1:250 Incubation time: overnight, 4C No permeabilization
    Cytokeratin, pan Antibody (PCK-26) NB120-6401
  • Name: Abhishek Aggarwal
    Application: Immunofluorescence
    Sample Tested: Prostate cancer (Human) cell line
    Species: Human
    Verified Customer | Posted 02/05/2013
    Pan cytokeratin in PNT2 cells
    Cytokeratin, pan Antibody (PCK-26) NB120-6401

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