D-Dimer Antibody (28) - BSA Free
Novus Biologicals | Catalog # NBP1-05045
![ELISA: D-Dimer Antibody (28) - BSA Free [NBP1-05045]](https://beta-resources.rndsystems.com/images/products/D-Dimer-Antibody-28-BSA-Free-ELISA-NBP1-05045-img0004.jpg "ELISA: D-Dimer Antibody (28) - BSA Free [NBP1-05045]")
Key Product Details
Species Reactivity
Validated:
Human, Canine
Cited:
Human
Applications
Validated:
Immunohistochemistry-Paraffin, Western Blot, ELISA
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 28
Format
BSA Free
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Product Specifications
Immunogen
Purified human D-dimer
Epitope
The epitope appears to relate to the neo-epitope formed by cleavage of D-dimer from fibrin.
Specificity
Binds human D-dimer. Cross-reacts with directly coated D-monomer but not with directly
coated fibrinogen.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Novus Biologicals Mouse D-Dimer Antibody (28) - BSA Free (NBP1-05045) is a monoclonal antibody validated for use in IHC, WB and ELISA. Anti-D-Dimer Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for D-Dimer Antibody (28) - BSA Free
ELISA: D-Dimer Antibody (28) - BSA Free [NBP1-05045]
ELISA: D-Dimer Antibody (28) - BSA Free [NBP1-05045] - The calibration curve of a sandwich assay for D-Dimer using NBP1-05045 as capture antibody and NBP2-23592 as the biotinylated detection antibody.Applications for D-Dimer Antibody (28) - BSA Free
Application
Recommended Usage
ELISA
1:8000
Western Blot
1:100-1:2000
Application Notes
ELISA: NBP1-05045 binds free D-dimer in solution. NBP1-05045 works as a capture antibody in sandwich ELISA with biotinylated NBP1-05045 or NBP2-21616 as a detection antibody. Biotinylated NBP1-05045 works as a detection antibody in sandwich ELISA with NBP2-21616 or NBP1-05045 as a capture antibody. The combination with NBP1-05045 as a capture antibody and NBP2-23592 as a detection antibody is recommended.
WB: Due to reducing conditions in Western blotting NBP1-05045 binds the beta-chain (40kDa) of D-Dimer, and not the entire D-Dimer complex. Use in IHC-P reported in scientific literature (PMID:33480806)
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: D-dimer
Long Name
D-Dimer breakdown product of Fibrinogen
Alternate Names
Ddimer, FDP
Additional D-dimer Products
Product Documents for D-Dimer Antibody (28) - BSA Free
Product Specific Notices for D-Dimer Antibody (28) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for D-Dimer Antibody (28) - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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