DAB/Ni Peroxidase Substrate Kit
Novus Biologicals | Catalog # SK-4100-NB
![Immunohistochemistry: DAB/Ni Peroxidase Substrate Kit [SK-4100-NB]](https://resources.rndsystems.com/images/products/DAB-Ni-Peroxidase-Substrate-Kit-Immunohistochemistry-SK-4100-NB-img0003.jpg "Immunohistochemistry: DAB/Ni Peroxidase Substrate Kit [SK-4100-NB]")
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Key Product Details
Species
Human
Applications
ELISA, Functional Assay, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Kit Type
Substrate Kit
Product Summary for DAB/Ni Peroxidase Substrate Kit
Features:
Greater sensitivity than conventional substrates.
Ideal for IHC/ICC/ISH and blots.
Heat Stable
Ideal for single and multiple labeling
Stock solutions supplied in convenient dropper bottles promoting ease of handling.
Includes nickel to produce a gray-black rather than brown reaction product.
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Product Specifications
Application Notes
.
Scientific Data Images for DAB/Ni Peroxidase Substrate Kit
Immunohistochemistry: DAB/Ni Peroxidase Substrate Kit [SK-4100-NB]
Immunohistochemistry: DAB/Ni Peroxidase Substrate Kit [SK-4100-NB] - Tumor tissue section showing specific cytoplasmic cell staining (brown, DAB) with hematoxylin QS (blue) counterstain.![Immunohistochemistry-Paraffin: DAB/Ni Peroxidase Substrate Kit [SK-4100-NB]](https://resources.rndsystems.com/images/products/DAB-Ni-Peroxidase-Substrate-Kit-Immunohistochemistry-Paraffin-SK-4100-NB-img0001.jpg "Immunohistochemistry-Paraffin: DAB/Ni Peroxidase Substrate Kit [SK-4100-NB]")
Immunohistochemistry-Paraffin: DAB/Ni Peroxidase Substrate Kit [SK-4100-NB]
Immunohistochemistry-Paraffin: DAB/Ni Peroxidase Substrate Kit [SK-4100-NB] - Paraffin-embedded human prostate tissue stained using rabbit anti-prostate specific antigen (PSA) detected with VECTASTAIN Elite ABC Reagent and Vector DAB substrate.
DAB/Ni Peroxidase Substrate Kit [SK-4100-NB]
Kit Contents for DAB/Ni Peroxidase Substrate Kit
- Buffer Stock Solution (6 mL)
- DAB Stock Solution (6 mL)
- Hydrogen Peroxide Solution (6 mL)
- Nickel Solution (6 mL)
Formulation, Preparation, and Storage
Concentration
Concentration is not relevant for this product. Please see the protocols for proper use of this product.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at 4C in the dark.
Product Documents for DAB/Ni Peroxidase Substrate Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for DAB/Ni Peroxidase Substrate Kit
This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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