Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Bovine (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DAD1 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 14 amino acids near the C-terminus of human DAD1. The immunogen is located within the last 50 amino acids of DAD1. Amino Acid Squence: CLRIQINPQNKAD

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (85%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DAD1 Antibody - BSA Free

Western Blot: DAD1 AntibodyBSA Free [NBP1-77197]

Western Blot: DAD1 AntibodyBSA Free [NBP1-77197]

Western Blot: DAD1 Antibody [NBP1-77197] - Analysis of DAD1 in HepG2 cell lysates with anti-DAD1 using this antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.
DAD1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: DAD1 Antibody - BSA Free [NBP1-77197] -

Immunocytochemistry/ Immunofluorescence: DAD1 Antibody - BSA Free [NBP1-77197] - Immunocytochemistry of DAD1 in HepG2 cells with DAD1 antibody at 10 ug/mL.
DAD1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: DAD1 Antibody - BSA Free [NBP1-77197] -

Immunocytochemistry/ Immunofluorescence: DAD1 Antibody - BSA Free [NBP1-77197] - Immunofluorescence of DAD1 in HepG2 cells with DAD1 antibody at 10 ug/mL.

Applications for DAD1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Western Blot

0.5-2 ug/ml
Application Notes
Despite its predicted molecular weight, DAD1 migrates at ~22 kDa in SDS-PAGE.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DAD1

Defender of cell death 1 (DAD1) was initially discovered in BHK21 cells as a negative regulator of programmed cell death, a process important for normal organism development and tissue homeostasis. DAD1 was later shown to be a subunit of the mammalian oligosaccharyltransferase complex and is required for its function and structural integrity. Mice lacking DAD1 express abnormal N-linked glycoproteins and undergo increased apoptotic-associated embryonic death. Furthermore, overexpression of DAD1 mRNA is seen in some human hepatocellular carcinomas, indicating it may also play a role in carcinogenesis. It should be noted that DAD1 is not related to the inhibitor of apoptosis proteins (IAP) family and does not contain any baculoviral IAP repeat (BIR) domains.

Alternate Names

DAD-1, defender against cell death 1EC 2.4.1.119, dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1, Oligosaccharyl transferase subunit DAD1, oligosaccharyltransferase 2 homolog, OST2

Gene Symbol

DAD1

UniProt

AAH09798 (Human)

Additional DAD1 Products

Product Documents for DAD1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for DAD1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for DAD1 Antibody - BSA Free

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Protocols

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