DAP Kinase 2 Antibody (OTI1C5)

Novus Biologicals | Catalog # NBP2-02477

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # OTI1C5
View all DAPK2 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human DAPK2 (NP_055141) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Theoretical MW

42.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DAP Kinase 2 Antibody (OTI1C5)

Western Blot: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477]

Western Blot: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477]

Western Blot: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-DAP Kinase 2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunocytochemistry/ Immunofluorescence: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477]

Immunocytochemistry/ Immunofluorescence: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477]

Immunocytochemistry/Immunofluorescence: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY DAP Kinase 2.
Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477]

Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477]

Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - Staining of paraffin-embedded Human pancreas tissue using anti-DAP Kinase 2 mouse monoclonal antibody.
Flow Cytometry: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477]

Flow Cytometry: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477]

Flow Cytometry: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DAP Kinase 2 antibody, and then analyzed by flow cytometry.
Western Blot: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477]

Western Blot: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477]

Western Blot: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DAP Kinase 2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DAP Kinase 2.
Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477]

Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477]

Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DAP Kinase 2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477]

Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477]

Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-DAP Kinase 2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477]

Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477]

Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - Staining of paraffin-embedded Human colon tissue using anti-DAP Kinase 2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477]

Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477]

Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - Staining of paraffin-embedded Human Kidney tissue using anti-DAP Kinase 2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477]

Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477]

Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - Staining of paraffin-embedded Human liver tissue using anti-DAP Kinase 2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477]

Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477]

Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - Staining of paraffin-embedded Human lung tissue using anti-DAP Kinase 2 mouse monoclonal antibody.
Flow Cytometry: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477]

Flow Cytometry: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477]

Flow Cytometry: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - Analysis of Jurkat cells, using anti-DAP Kinase 2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Immunoprecipitation: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477]

Immunoprecipitation: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477]

Immunoprecipitation: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - (Negative control: IP without adding anti-DAPK2 antibody.). For each experiment, 500ul of DDK tagged DAPK2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-DAPK2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Applications for DAP Kinase 2 Antibody (OTI1C5)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Immunoprecipitation

2ug/500ul

Western Blot

1:1000-2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.76 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DAPK2

This gene encodes a protein that belongs to the serine/threonine protein kinase family. This protein contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain with significant similarity to that of death-associated protein kinase 1 (DAPK1), a positive regulator of programmed cell death. Overexpression of this gene was shown to induce cell apoptosis. It uses multiple polyadenylation sites. [provided by RefSeq].

Long Name

Death-associated Protein Kinase 2

Alternate Names

DAP Kinase 2, DRP-1

Entrez Gene IDs

23604 (Human)

Gene Symbol

DAPK2

Additional DAPK2 Products

Product Documents for DAP Kinase 2 Antibody (OTI1C5)

Certificate of Analysis

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Product Specific Notices for DAP Kinase 2 Antibody (OTI1C5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Intracellular Kinases

Citations for DAP Kinase 2 Antibody (OTI1C5)

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Protocols

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