DAP3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88568

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DAP3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GSPLGEVVEQGITRVRNATDAVGIVLKELKRQSSLGMFHLLVAVDGINALWGRTTLKREDKSPIAPEELALVHNLRKMMKNDWHGGAIVSALSQTGSLFKPRKAYLPQELLGKEGFDALDPFIPILVSNYNPKEF

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DAP3 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: DAP3 Antibody [NBP1-88568]

Immunocytochemistry/ Immunofluorescence: DAP3 Antibody [NBP1-88568]

Immunocytochemistry/Immunofluorescence: DAP3 Antibody [NBP1-88568] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & mitochondria.
Immunohistochemistry-Paraffin: DAP3 Antibody [NBP1-88568]

Immunohistochemistry-Paraffin: DAP3 Antibody [NBP1-88568]

Immunohistochemistry-Paraffin: DAP3 Antibody [NBP1-88568] - Staining of human colon shows moderate to strong positivity in mitochondria in glandular cells.
Immunohistochemistry-Paraffin: DAP3 Antibody [NBP1-88568]

Immunohistochemistry-Paraffin: DAP3 Antibody [NBP1-88568]

Immunohistochemistry-Paraffin: DAP3 Antibody [NBP1-88568] - Staining of human cerebral cortex shows weak to moderate cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: DAP3 Antibody [NBP1-88568]

Immunohistochemistry-Paraffin: DAP3 Antibody [NBP1-88568]

Immunohistochemistry-Paraffin: DAP3 Antibody [NBP1-88568] - Staining of human prostate shows moderate to strong positivity in mitochondria in glandular cells.
Immunohistochemistry-Paraffin: DAP3 Antibody [NBP1-88568]

Immunohistochemistry-Paraffin: DAP3 Antibody [NBP1-88568]

Immunohistochemistry-Paraffin: DAP3 Antibody [NBP1-88568] - Staining of human duodenum shows moderate to strong positivity in mitochondria in glandular cells.
DAP3 Antibody - BSA Free Western Blot: DAP3 Antibody - BSA Free [NBP1-88568]

Western Blot: DAP3 Antibody - BSA Free [NBP1-88568]

Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
DAP3 Antibody - BSA Free Western Blot: DAP3 Antibody - BSA Free [NBP1-88568]

Western Blot: DAP3 Antibody - BSA Free [NBP1-88568]

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp

Applications for DAP3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DAP3

Death Associated Protein 3 (DAP3) is a 46 kDa protein carrying a potential nucleotide binding motif (P-loop) at its N-terminal region. The protein is widely expressed, and the expression is upregulated during membrane-receptor mediated apoptosis. In interferon-gamma and Fas induced apoptosis, DAP3 acts as a positive mediator by functioning downstream of the receptor signaling complex and upstream of the effector caspases. However, the mechanism for its function is largely unknown. Interestingly, the N-terminal 230 amino acid fragment of DAP3 alone protects cells from Fas induced cell death. The same region of DAP3 also serves as a binding site for the glucocorticoid receptor and mediates receptor functions.

Alternate Names

bMRP-10, DAP-3Death-associated protein 3, death associated protein 3, DKFZp686G12159,28S ribosomal protein S29, mitochondrial, FLJ12817, MGC126058, MGC126059, mitochondrial 28S ribosomal protein S29, MRP-S29Ionizing radiation resistance conferring protein, MRPS29S29mt

Gene Symbol

DAP3

Additional DAP3 Products

Product Documents for DAP3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for DAP3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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