DARC Antibody (2C3) - Chimeric - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-75197

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Block/Neutralize, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # 2C3

Format

Azide and BSA Free
View all DARC Primary Antibodies »

Product Specifications

Immunogen

CHO cells transfected with cDNA encoding the Duffy glycoprotein.

Specificity

The antibody binds specifcally to the pentapeptide FEDVW of the Duffy receptor.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for DARC Antibody (2C3) - Chimeric - Azide and BSA Free

Western Blot: DARC Antibody (2C3)ChimericAzide and BSA Free [NBP2-75197]

Western Blot: DARC Antibody (2C3)ChimericAzide and BSA Free [NBP2-75197]

Western Blot: DARC Antibody (2C3) - Chimeric [NBP2-75197] - Western Blot using anti-DARC antibody 2C3 (Ab00893) Human liver lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 2C3 (NBP2-75197) at 0.03 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for DARC is 35.6kDa. NBP2-75197 successfully detected DARC in human liver lysate.
DARC Antibody (2C3) - Chimeric - Azide and BSA Free

Immunocytochemistry /Immunofluorescence: DARC Antibody (2C3) - Chimeric - Azide and BSA Free [NBP2-75197] -

Immunocytochemistry /Immunofluorescence: DARC Antibody (2C3) - Chimeric - Azide and BSA Free [NBP2-75197] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells on coverslips, permeabilized with 0.15% Triton and stained with NBP2-75197 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic staining, likely of endosomes, and some membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-75197, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.

Applications for DARC Antibody (2C3) - Chimeric - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Flow Cytometry

1:10 - 1:1000
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: DARC

DARC is a non-specific receptor for many chemokines such as IL-8, GRO, RANTES, MCP-1 and TARC. It is also the receptor for the human malaria parasites Plasmodium vivax and Plasmodium knowlesi.

Long Name

Duffy Antigen, Receptor For Chemokines

Alternate Names

ACKR1, CD234, Duffy Antigen, Fy Antigen, GPD, GpFy

Gene Symbol

ACKR1

Additional DARC Products

Product Documents for DARC Antibody (2C3) - Chimeric - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for DARC Antibody (2C3) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Red Blood Cells (RBCs)

Citations for DARC Antibody (2C3) - Chimeric - Azide and BSA Free

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Protocols

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