DARS Antibody (2F11) - Azide and BSA Free

Novus Biologicals | Catalog # H00001615-M01

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2F11

Format

Azide and BSA Free
View all DARS Primary Antibodies »

Product Specifications

Immunogen

DARS (NP_001340, 393 a.a. ~ 500 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KYPLAVRPFYTMPDPRNPKQSNSYDMFMRGEEILSGAQRIHDPQLLTERALHHGIDLEKIKAYIDSFRFGAPPHAGGGIGLERVTMLFLGLHNVRQTSMFPRDPKRLT

Specificity

DARS - aspartyl-tRNA synthetase

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for DARS Antibody (2F11) - Azide and BSA Free

Western Blot: DARS Antibody (2F11) [H00001615-M01]

Western Blot: DARS Antibody (2F11) [H00001615-M01]

Western Blot: DARS Antibody (2F11) [H00001615-M01] - Analysis of DARS expression in transfected 293T cell line by DARS monoclonal antibody (M01), clone 2F11.Lane 1: DARS transfected lysate(57.1 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: DARS Antibody (2F11) [H00001615-M01]

Immunohistochemistry-Paraffin: DARS Antibody (2F11) [H00001615-M01]

Immunohistochemistry-Paraffin: DARS Antibody (2F11) [H00001615-M01] - Analysis of monoclonal antibody to DARS on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.
Western Blot: DARS Antibody (2F11) [H00001615-M01]

Western Blot: DARS Antibody (2F11) [H00001615-M01]

Western Blot: DARS Antibody (2F11) [H00001615-M01] - Analysis of DARS over-expressed 293 cell line, cotransfected with DARS Validated Chimera RNAi ( Cat # H00001615-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with DARS monoclonal antibody (M01), clone 2F11 (Cat # H00001615-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: DARS Antibody (2F11) [H00001615-M01]

Western Blot: DARS Antibody (2F11) [H00001615-M01]

Western Blot: DARS Antibody (2F11) [H00001615-M01] - DARS monoclonal antibody (M01), clone 2F11 Analysis of DARS expression in HeLa.
Sandwich ELISA: DARS Antibody (2F11) [H00001615-M01] - Detection limit for recombinant GST tagged DARS is approximately 0.3ng/ml as a capture antibody.

Applications for DARS Antibody (2F11) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P, RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DARS

Aspartyl-tRNA synthetase (DARS) is part of a multienzyme complex of aminoacyl-tRNA synthetases. Aspartyl-tRNA synthetase charges its cognate tRNA with aspartate during protein biosynthesis.

Alternate Names

aspartate tRNA ligase 1, cytoplasmic, Aspartate--tRNA ligase, aspartyl-tRNA synthetase, aspartyl-tRNA synthetase, cytoplasmic, AspRS, Cell proliferation-inducing gene 40 protein, cell proliferation-inducing protein 40, DKFZp781B11202, EC 6.1.1.12, MGC111579

Entrez Gene IDs

1615 (Human)

Gene Symbol

DARS1

OMIM

603084 (Human)

UniProt

NP_001340 (Human)

Additional DARS Products

Product Documents for DARS Antibody (2F11) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for DARS Antibody (2F11) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for DARS Antibody (2F11) - Azide and BSA Free

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Protocols

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FAQs

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