Daxx Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85309

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Daxx Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DEEEEAAAGKDGDKSPMSSLQISNEKNLEPGKQISRSSGEQQNKGRIVSPSLLSEEPLAPSSIDAESNGEQPEELTLEEESPVSQLFELEIEALPLDTPSSVETDISSSRKQSEEPFTTVLENGAGMVSSTSFNGGVSPHNWGDSG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Daxx Antibody - BSA Free

Western Blot: Daxx Antibody [NBP1-85309]

Western Blot: Daxx Antibody [NBP1-85309]

Western Blot: Daxx Antibody [NBP1-85309] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-DAXX antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunohistochemistry-Paraffin: Daxx Antibody [NBP1-85309]

Immunohistochemistry-Paraffin: Daxx Antibody [NBP1-85309]

Immunohistochemistry-Paraffin: Daxx Antibody [NBP1-85309] - Staining in human testis and skeletal muscle tissues. Corresponding DAXX RNA-seq data are presented for the same tissues.
Western Blot: Daxx Antibody [NBP1-85309]

Western Blot: Daxx Antibody [NBP1-85309]

Western Blot: Daxx Antibody [NBP1-85309] - Analysis in control (vector only transfected HEK293T lysate) and DAXX over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: Daxx Antibody [NBP1-85309]

Immunohistochemistry-Paraffin: Daxx Antibody [NBP1-85309]

Immunohistochemistry-Paraffin: Daxx Antibody [NBP1-85309] - Staining of human fallopian tube shows moderate to strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: Daxx Antibody [NBP1-85309]

Immunohistochemistry-Paraffin: Daxx Antibody [NBP1-85309]

Immunohistochemistry-Paraffin: Daxx Antibody [NBP1-85309] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: Daxx Antibody [NBP1-85309]

Immunohistochemistry-Paraffin: Daxx Antibody [NBP1-85309]

Immunohistochemistry-Paraffin: Daxx Antibody [NBP1-85309] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts and in Leydig cells.
Immunohistochemistry-Paraffin: Daxx Antibody [NBP1-85309]

Immunohistochemistry-Paraffin: Daxx Antibody [NBP1-85309]

Immunohistochemistry-Paraffin: Daxx Antibody [NBP1-85309] - Staining of human tonsil shows moderate to strong nuclear positivity in lymphoid cells.

Applications for Daxx Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Daxx

Daxx (death-domain-associated protein) was originally identified as a cytoplasmic protein that binds to the death domain of the transmembrane death receptor Fas. It is now that known that the a large porportion of Daxx molecules are nuclear and associate with the promyelocytic leukaemia nuclear body (PML-NB) and other subnuclear domains (reviewed in Salomoni and Khelifi). The promyelocytic leukemia protein Pml is an essential component of the PML-NB. Data suggests that certain stimuli including Fas stimulation, causes Daxx to be translocated from the nucleus to the cytoplasm. Daxx is ubiquitously expressed, and particularly high levels of Daxx have been reported in the thymus and testes. At the cellular level Daxx may be found in cytoplasm and within heterochromatic regions of the nucleus. Daxx has been reported to interact and co-localize with Pml in the nucleus of tumor cell lines and primary cells. Pml is necessary for Fas-induced cell death and Daxx pro-apoptotic functions. Daxx is thought to have both pro- and anti-apoptotic functions depending on the stimulus and the cell type. Daxx pro-apoptotic functions appear to occur in both the cytoplasm and nucleus. Although the mechanisms remain to be fully elucidated, research indicates that Daxx plays a role in the pathology of human diseases including cancer and neurodegerative disorders. Human Daxx is a 740 amino acid protein (GenBank no. gi/62898369).

Long Name

Fas Death Domain-associated Protein

Alternate Names

BING2, DAP6

Gene Symbol

DAXX

Additional Daxx Products

Product Documents for Daxx Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Daxx Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Apoptosis Adaptor Proteins

Citations for Daxx Antibody - BSA Free

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Protocols

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