DBC1 Antibody

Novus Biologicals | Catalog # NBP3-48524

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all DBC1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human DBC1. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DBC1 Antibody

DBC1 Antibody

Immunohistochemistry: DBC1 Antibody [NBP3-48524] -

DBC1 antibody [N1N2], N-term detects DBC1 protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
DBC1 antibody [N1N2], N-term (NBP3-48524) dilution: 1:500.


Antigen Retrieval: (EDTA based, pH 8.0) buffer, 15min
DBC1 Antibody

Immunohistochemistry: DBC1 Antibody [NBP3-48524] -

DBC1 antibody [N1N2], N-term detects DBC1 protein at nucleus on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
DBC1 antibody [N1N2], N-term (NBP3-48524) dilution: 1:500.


Antigen Retrieval: (EDTA based, pH 8.0) buffer, 15min
DBC1 Antibody

Immunocytochemistry/ Immunofluorescence: DBC1 Antibody [NBP3-48524] -

DBC1 antibody [N1N2], N-term detects DBC1 protein at cytoplasm by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 100% MeOH for 5 min.
Green: DBC1 protein stained by DBC1 antibody [N1N2], N-term (NBP3-48524) diluted at 1:500.
Blue: Hoechst 33342 staining.
DBC1 Antibody

Western Blot: DBC1 Antibody [NBP3-48524] -

DBC1 antibody detects DBC1 protein by Western blot analysis.
A. 30 ug MCF-7 whole cell lysate/extract
7.5 % SDS-PAGE
DBC1 antibody (NBP3-48524) dilution: 1:500

Applications for DBC1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DBC1

The DBC1 gene is located within a chromosomal region that shows loss of heterozygosity in some bladder cancers. It containsa 5' CpG island that may be a frequent target of hypermethylation, and it may undergo hypermethylation-basedsilencing in some bladder ca

Alternate Names

DBCCR1deleted in bladder cancer protein 1, deleted in bladder cancer 1, deleted in bladder cancer chromosome region candidate 1, FAM5AbA574M5.1 (deleted in bladder cancer chromosome region candidate 1 (IB3089A)), Protein FAM5A

Gene Symbol

BRINP1

Additional DBC1 Products

Product Documents for DBC1 Antibody

Certificate of Analysis

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Product Specific Notices for DBC1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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