Dcp1a Antibody (7Q8Q6)

Novus Biologicals | Catalog # NBP3-16644

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7Q8Q6 expressed in HEK293
View all Dcp1a Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 483-582 of human DCP1A (Q9NPI6). PVAGAPLVTATTTAVSSVLLAPSVFQQTVTRSSDLERKASSPSPLTIGTPESQRKPSIILSKSQLQDTLIHLIKNDSSFLSTLHEVYLQVLTKNKDNHNL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Dcp1a Antibody (7Q8Q6)

Western Blot: Dcp1a Antibody (7Q8Q6) [NBP3-16644]

Western Blot: Dcp1a Antibody (7Q8Q6) [NBP3-16644]

Western Blot: Dcp1a Antibody (7Q8Q6) [NBP3-16644] - Western blot analysis of extracts of various cell lines, using Dcp1a Rabbit mAb (NBP3-16644) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunocytochemistry/ Immunofluorescence: Dcp1a Antibody (7Q8Q6) [NBP3-16644]

Immunocytochemistry/ Immunofluorescence: Dcp1a Antibody (7Q8Q6) [NBP3-16644]

Immunocytochemistry/Immunofluorescence: Dcp1a Antibody (7Q8Q6) [NBP3-16644] - Immunofluorescence analysis of U-2 OS cells using Dcp1a Rabbit mAb (NBP3-16644) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: Dcp1a Antibody (7Q8Q6) [NBP3-16644]

Immunohistochemistry-Paraffin: Dcp1a Antibody (7Q8Q6) [NBP3-16644]

Immunohistochemistry-Paraffin: Dcp1a Antibody (7Q8Q6) [NBP3-16644] - Immunohistochemistry of paraffin-embedded human esophageal cancer using Dcp1a Rabbit mAb (NBP3-16644) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence: Dcp1a Antibody (7Q8Q6) [NBP3-16644]

Immunocytochemistry/ Immunofluorescence: Dcp1a Antibody (7Q8Q6) [NBP3-16644]

Immunocytochemistry/Immunofluorescence: Dcp1a Antibody (7Q8Q6) [NBP3-16644] - Immunofluorescence analysis of NIH-3T3 cells using Dcp1a Rabbit mAb (NBP3-16644) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Dcp1a Antibody (7Q8Q6)

Immunocytochemistry/ Immunofluorescence: Dcp1a Antibody (7Q8Q6) [NBP3-16644] -

Immunocytochemistry/ Immunofluorescence: Dcp1a Antibody (7Q8Q6) [NBP3-16644] - Confocal imaging of NIH/3T3 cells using Dcp1a Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Applications for Dcp1a Antibody (7Q8Q6)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Dcp1a

Decapping is a key step in general and regulated mRNA decay. The protein encoded by this gene is a decapping enzyme. This protein and another decapping enzyme form a decapping complex, which interacts with the nonsense-mediated decay factor hUpf1 and may be recruited to mRNAs containing premature termination codons. This protein also participates in the TGF-beta signaling pathway.

Alternate Names

DCP1 decapping enzyme homolog A (S. cerevisiae), decapping enzyme hDcp1a, EC 3.-, FLJ21691, mRNA-decapping enzyme 1A, Nbla00360, putative protein product of Nbla00360, Smad4-interacting transcriptional co-activator, SMAD4IP1, SMIFHSA275986, Transcription factor SMIF

Gene Symbol

DCP1A

Additional Dcp1a Products

Product Documents for Dcp1a Antibody (7Q8Q6)

Certificate of Analysis

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Product Specific Notices for Dcp1a Antibody (7Q8Q6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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