Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Bovine (100%), Canine (100%), Chimpanzee (100%), Equine (100%), Monkey (100%), Orangutan (100%), Primate (100%), Rabbit (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human protein regulated in development and DNA damage response 1 (DNA-damage-inducible transcript 4) using the numbering given in entry NP_061931.1 (GeneID 54541).

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25867045)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DDIT4 Antibody - BSA Free

Western Blot: DDIT4 Antibody [NBP1-22966]

Western Blot: DDIT4 Antibody [NBP1-22966]

DDIT4-Antibody-Western-Blot-NBP1-22966-img0005.jpg
Immunocytochemistry/ Immunofluorescence: DDIT4 Antibody [NBP1-22966]

Immunocytochemistry/ Immunofluorescence: DDIT4 Antibody [NBP1-22966]

Immunocytochemistry/Immunofluorescence: DDIT4 Antibody [NBP1-22966] - HeLa cells stained with NBP1-22966 after 0.2% hypoxia or CoCl2 overnight. Image from verified customer review.
Western Blot: DDIT4 Antibody [NBP1-22966]

Western Blot: DDIT4 Antibody [NBP1-22966]

Western Blot: DDIT4 Antibody [NBP1-22966] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Lysate was prepared from cells that had been treated with cobalt chloride (A and B) or mock treated (A). NBP1-22966 used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate.
Western Blot: DDIT4 Antibody [NBP1-22966]

Western Blot: DDIT4 Antibody [NBP1-22966]

DDIT4-Antibody-Western-Blot-NBP1-22966-img0006.jpg
Immunocytochemistry/ Immunofluorescence: DDIT4 Antibody [NBP1-22966]

Immunocytochemistry/ Immunofluorescence: DDIT4 Antibody [NBP1-22966]

Immunocytochemistry/Immunofluorescence: DDIT4 Antibody [NBP1-22966] - NBF-fixed asynchronous, cobalt-treated HeLa cells. Antibody: Affinity purified rabbit anti-REDD1 used at a dilution of 1:500 (2 ug/ml). Detection: Red-fluorescent goat anti-rabbit IgG H&L crossadsorbed Antibody DyLight®594 used at 1:100.

Applications for DDIT4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500 to 1:2000

Immunoprecipitation

5-15 ug/mg lysate

Western Blot

1:1000-1:4000
Application Notes
Formaldehyde fixation is recommended. Permeabilization with Triton X-100 is recommended for formaldehydefixed cells.

Reviewed Applications

Read 1 review rated 4 using NBP1-22966 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DDIT4

REDD1 (regulated in development and DNA damage response 1) is a direct transcriptional target of HIF. It plays a role in the TSC/mTOR pathway as a regulator upstream of the TSC1/2 complex. In response to both hypoxia and energy stress, REDD1 inhibits mTOR activity in a TSC-dependent manner.

Alternate Names

Dig2, DNA-damage-inducible transcript 4, FLJ20500, HIF-1 responsive RTP801 (RTP801), Protein regulated in development and DNA damage response 1, REDD-1DNA damage-inducible transcript 4 protein, REDD1HIF-1 responsive protein RTP801, RTP801RP11-442H21.1

Entrez Gene IDs

54541 (Human)

Gene Symbol

DDIT4

UniProt

Q9NX09 (Human)

Additional DDIT4 Products

Product Documents for DDIT4 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for DDIT4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for DDIT4 Antibody - BSA Free

Customer Reviews for DDIT4 Antibody - BSA Free (1)

4 out of 5
1 Customer Rating
5 Stars
0%
4 Stars
100%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

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  • DDIT4 Antibody
    Name: Natalie Erdmann
    Application: Immunocytochemistry
    Sample Tested: hela cell
    Species: Human
    Verified Customer | Posted 05/24/2019
    Immunofluorescence/Immunocytochemistry: HeLa stained with NBP1-22966 after 0.2% hypoxia or CoCl2 overnight.
    HeLa cells treated with 0.2% hypoxia or different doses CoCl2 overnight; fixed in 4% PFA 10 minutes (directly in hypoxia chamber or quickly after washing off CoCl2/media as half-life DDIT4 is 5-10 minutes); permeabilize 0.5% Triton X-100 5 minutes; dilute primary antibody 1/500 incubate O/N @4oC; wash 3X PBS; Alexa 488 donkey anti-rabbit F(ab”)2 secondary antibody 1/1000 1 hour at rt; wash 5X PBS; mount DAPI/Vectashield; images acquired on Olympus BX63 microscope with Bioview Allegro software.
    DDIT4 Antibody - BSA Free NBP1-22966

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