DDR2 Antibody

Novus Biologicals | Catalog # NBP2-14926

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all DDR2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human DDR2. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DDR2 Antibody

Immunohistochemistry-Paraffin: DDR2 Antibody [NBP2-14926]

Immunohistochemistry-Paraffin: DDR2 Antibody [NBP2-14926]

Immunohistochemistry-Paraffin: DDR2 Antibody [NBP2-14926] - Human breast cancer, using DDR2 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Western Blot: DDR2 Antibody [NBP2-14926]

Western Blot: DDR2 Antibody [NBP2-14926]

Western Blot: DDR2 Antibody [NBP2-14926] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DDR2 antibody diluted at 1:3000.
Western Blot: DDR2 Antibody [NBP2-14926]

Western Blot: DDR2 Antibody [NBP2-14926]

Western Blot: DDR2 Antibody [NBP2-14926] - U87-MG whole cell and membrane extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DDR2 antibody diluted at 1:1000.
DDR2 Antibody

Western Blot: DDR2 Antibody [NBP2-14926] -

Western Blot: DDR2 Antibody [NBP2-14926] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with DDR2 antibody [C2C3], C-term (NBP2-14926) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
DDR2 Antibody

Immunocytochemistry/ Immunofluorescence: DDR2 Antibody [NBP2-14926] -

DDR2 antibody [C2C3], C-term detects DDR2 protein at cell membrane by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DDR2 stained by DDR2 antibody [C2C3], C-term (NBP2-14926) diluted at 1:250.
Red: Tau, an Axon marker, stained by Tau antibody [GT287] diluted at 1:500.
Blue: Fluoroshield with DAPI.
DDR2 Antibody

Western Blot: DDR2 Antibody [NBP2-14926] -

Wild-type (WT) and DDR2 (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DDR2 antibody [C2C3], C-term (NBP2-14926) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for DDR2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Assay dependent

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DDR2

Receptor tyrosine kinases (RTKs) play a key role in the communication of cells with their microenvironment. These molecules are involved in the regulation of cell growth, differentiation, and metabolism. In several cases the biochemical mechanism by which RTKs transduce signals across the membrane has been shown to be ligand induced receptor oligomerization and subsequent intracellular phosphorylation. This autophosphorylation leads to phosphorylation of cytosolic targets as well as association with other molecules, which are involved in pleiotropic effects of signal transduction. RTKs have a tripartite structure with extracellular, transmembrane, and cytoplasmic regions. This gene encodes a member of a novel subclass of RTKs and contains a distinct extracellular region encompassing a factor VIII-like domain. Alternative splicing in the 5' UTR results in multiple transcript variants encoding the same protein. [provided by RefSeq]

Long Name

Discoidin Domain Receptor 2

Alternate Names

TKT, Trk3, Tyro-10

Gene Symbol

DDR2

UniProt

Q16832

Additional DDR2 Products

Product Documents for DDR2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for DDR2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Receptor Tyrosine Kinases (RTKs)

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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Associated Pathways

Articular Cartilage Extracellular Matrix Articular Cartilage Extracellular Matrix Thumbnail