DDX1 Antibody

Novus Biologicals | Catalog # NB200-349

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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Chicken (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all DDX1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 650 and the C-terminus (residue 697) of human DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 1 using the numbering given in entry AAH12739.1 (GeneID 1653).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DDX1 Antibody

Western Blot: DDX1 Antibody [NB200-349]

Western Blot: DDX1 Antibody [NB200-349]

Western Blot: DDX1 Antibody [NB200-349] - Detection of Human and Mouse DDX1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DDX1 antibody NB200-349 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.
Immunohistochemistry: DDX1 Antibody [NB200-349]

Immunohistochemistry: DDX1 Antibody [NB200-349]

Immunohistochemistry: DDX1 Antibody [NB200-349] - Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-DDX1 used at a dilution of 1:200 ( 1ug/ml). Detection: DAB
Western Blot: DDX1 Antibody [NB200-349]

Western Blot: DDX1 Antibody [NB200-349]

Western Blot: DDX1 Antibody [NB200-349] - HeLa whole cell lysate. Antibody: NB200-349 used at 0.3 ug/ml for WB (A & B) and at 3 ug/ml lysate for IP (B).
Immunohistochemistry: DDX1 Antibody [NB200-349]

Immunohistochemistry: DDX1 Antibody [NB200-349]

Immunohistochemistry: DDX1 Antibody [NB200-349] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-DDX1 used at a dilution of 1:200 ( 1ug/ml). Detection: DAB
Immunoprecipitation: DDX1 Antibody [NB200-349]

Immunoprecipitation: DDX1 Antibody [NB200-349]

Immunoprecipitation: DDX1 Antibody [NB200-349] - Detection of human DDX1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DDX1 antibody NB200-349 (lot NB200-349-2) used for IP at 6 ug per reaction. DDX1 was also immunoprecipitated by a previous lot of this antibody (lot NB200-349-1). For blotting immunoprecipitated DDX1, NB200-349 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.

Applications for DDX1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

3-5 ug/mg lysate

Western Blot

1:1000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DDX1

DEAD box proteins, a family of putative RNA helicases, are characterized by eight conserved amino acid motifs that are arranged in a core region as found in the prototypical member of the family, eIF-4A. The family's name is derived from the amino acid sequence Asp-Glu-Ala-Asp (DEAD) that is located within the ATP hydrolysis motif. DEAD box proteins have been implicated in translation initiation and RNA splicing, degradation, and stability. The DEAD box protein, DDX1, was identified in retinoblastoma cell lines, where it is coamplified with MYCN, a member of the MYC transcription factor family. DDX1 is expressed at elevated levels in fetal tissues of neural origin (retina and brain). It is both cytoplasmic and nuclear in DDX1-amplified neuroblastoma and retinoblastoma cell lines. However, it localizes specifically to the nucleus in non-amplified cell lines. Thus, DDX1 is thought to be involved in nuclear RNA metabolism. In addition, the decrease in mean disease-free survival of neuroblastoma patients exhibiting coamplification of DDX1 and MYCN as compared to those exhibiting MYCN amplification indicates a possible role for DDX1 in cancer formation in neural tissues.

Long Name

DEAD-Box Helicase 1

Alternate Names

DBP-RB, DEAD Box-1, DEAD/H-Box Helicase 1, UKVH5d

Entrez Gene IDs

1653 (Human)

Gene Symbol

DDX1

UniProt

O72127 (Virus)

Additional DDX1 Products

Product Documents for DDX1 Antibody

Certificate of Analysis

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Product Specific Notices for DDX1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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