DDX1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85293

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DDX1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LGKSHIRTDDVHAKDNTRPGANSPEMWSEAIKILKGEYAVRAIKEHKMDQAIIFCRTKIDCDNLEQYFIQQGGGPDKKGHQFSCVCLHGD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DDX1 Antibody - BSA Free

Western Blot: DDX1 Antibody [NBP1-85293]

Western Blot: DDX1 Antibody [NBP1-85293]

Western Blot: DDX1 Antibody [NBP1-85293] - Analysis using Anti-DDX1 antibody NBP1-85293 (A) shows similar pattern to independent antibody NBP1-85294 (B).
Immunocytochemistry/ Immunofluorescence: DDX1 Antibody [NBP1-85293]

Immunocytochemistry/ Immunofluorescence: DDX1 Antibody [NBP1-85293]

Immunocytochemistry/Immunofluorescence: DDX1 Antibody [NBP1-85293] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: DDX1 Antibody [NBP1-85293]

Immunohistochemistry-Paraffin: DDX1 Antibody [NBP1-85293]

Immunohistochemistry-Paraffin: DDX1 Antibody [NBP1-85293] - Staining of human colon.
Western Blot: DDX1 Antibody [NBP1-85293]

Western Blot: DDX1 Antibody [NBP1-85293]

Western Blot: DDX1 Antibody [NBP1-85293] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunohistochemistry-Paraffin: DDX1 Antibody [NBP1-85293]

Immunohistochemistry-Paraffin: DDX1 Antibody [NBP1-85293]

Immunohistochemistry-Paraffin: DDX1 Antibody [NBP1-85293] - Staining of human colon shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: DDX1 Antibody [NBP1-85293]

Immunohistochemistry-Paraffin: DDX1 Antibody [NBP1-85293]

Immunohistochemistry-Paraffin: DDX1 Antibody [NBP1-85293] - Staining of human cerebral cortex, colon, kidney and testis using Anti-DDX1 antibody NBP1-85293 (A) shows similar protein distribution across tissues to independent antibody NBP1-85294 (B).
Immunohistochemistry-Paraffin: DDX1 Antibody [NBP1-85293]

Immunohistochemistry-Paraffin: DDX1 Antibody [NBP1-85293]

Immunohistochemistry-Paraffin: DDX1 Antibody [NBP1-85293] - Staining of human kidney.
Immunohistochemistry-Paraffin: DDX1 Antibody [NBP1-85293]

Immunohistochemistry-Paraffin: DDX1 Antibody [NBP1-85293]

Immunohistochemistry-Paraffin: DDX1 Antibody [NBP1-85293] - Staining of human cerebral cortex.
Immunohistochemistry-Paraffin: DDX1 Antibody [NBP1-85293]

Immunohistochemistry-Paraffin: DDX1 Antibody [NBP1-85293]

Immunohistochemistry-Paraffin: DDX1 Antibody [NBP1-85293] - Staining of human testis.

Applications for DDX1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DDX1

DDX1, also known as ATP-dependent RNA helicase DDX1, is an 82.4 kDa 740 amino acid protein and is involved in unwinding RNA helixes and in altering RNA secondary structure. Current research is being conducted for diseases and disorders such as carcinoma, malaria, hepatitis, neuroblastoma, heavy chain disease, prostate cancer, and breast cancer. The protein is involved in mRNA processing pathways with other proteins such as HNRNPK, NBN, SRRM2, RELB, and ATM.

Long Name

DEAD-Box Helicase 1

Alternate Names

DBP-RB, DEAD Box-1, DEAD/H-Box Helicase 1, UKVH5d

Gene Symbol

DDX1

Additional DDX1 Products

Product Documents for DDX1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for DDX1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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