DDX24 Antibody

Novus Biologicals | Catalog # NB100-2226

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Predicted:

Orangutan (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all DDX24 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 800 and the C-terminus (residue 859) of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 24 using the numbering given in entry NP_065147.1 (GeneID 57062).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DDX24 Antibody

Western Blot: DDX24 Antibody [NB100-2226]

Western Blot: DDX24 Antibody [NB100-2226]

Western Blot: DDX24 Antibody [NB100-2226] - Detection of Human DDX24 on HeLa whole cell lystate using NB100-2226. DDX24 was also immunoprecipitated by rabbit anti-DDX24 antibody NB100-2225 and NB100-2225.
Immunohistochemistry-Paraffin: DDX24 Antibody [NB100-2226]

Immunohistochemistry-Paraffin: DDX24 Antibody [NB100-2226]

Immunohistochemistry-Paraffin: DDX24 Antibody [NB100-2226] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-DDX24 used at a dilution of 1:200 ( 1ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: DDX24 Antibody [NB100-2226]

Immunohistochemistry-Paraffin: DDX24 Antibody [NB100-2226]

Immunohistochemistry-Paraffin: DDX24 Antibody [NB100-2226] - Staining of paraffin embedded human liver tissue with at a dilution of 1:100, detected using HRP/DAB detection system, counterstained with hematoxylin. Epitope retrieval, HIER with Tris/EDTA (pH 9.0) buffer using pressure cooker for 20 mins. Diluted using TBS with 5% BSA and incubated at 4 degrees C overnight. Image submitted by a verified customer review.

Applications for DDX24 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. DDX24 antibody validated for IHC-P from a verified customer review.

Reviewed Applications

Read 1 review rated 4 using NB100-2226 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DDX24

DDX24 is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. The specific function of DDX24 has not been elucidated. Alternate names for DDX24 include ATP-dependent RNA helicase DDX24, and DEAD box protein 24.

Alternate Names

ATP-dependent RNA helicase DDX24, DEAD (Asp-Glu-Ala-Asp) box polypeptide 24, DEAD box protein 24, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 24, EC 3.6.1, EC 3.6.4.13, S. cerevisiae CHL1-like helicase

Entrez Gene IDs

57062 (Human)

Gene Symbol

DDX24

UniProt

NP_065147.1 (Human)

Additional DDX24 Products

Product Documents for DDX24 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for DDX24 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for DDX24 Antibody (1)

4 out of 5
1 Customer Rating
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4 Stars
100%
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Customer Images

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  • DDX24 Antibody
    Name: Haopei Zhang
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Liver
    Species: Human
    Verified Customer | Posted 02/21/2019
    Immunohistochemical staining of paraffin embedded human liver tissue with anti-DDX24 [NB100-2226] at a dilution of 1:100, detected using HRP/DAB detection system, counterstained with hematoxylin.
    Epitope retrieval: HIER with Tris/EDTA (pH=9.0) buffer using pressure cooker for 20 mins. Primary antibody: Diluted using TBS with 5% BSA at 1/100, incubate at 4°C overnight. Detection system: HRP/DAB
    DDX24 Antibody NB100-2226

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Protocols

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FAQs

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