DDX3 Antibody

Novus Biologicals | Catalog # NB200-194

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all DDX3 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 3 using the numbering given in entry NP_001347.2 (GeneID 1654).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DDX3 Antibody

Western Blot: DDX3 Antibody [NB200-194]

Western Blot: DDX3 Antibody [NB200-194]

Western Blot: DDX3 Antibody [NB200-194] - Detection of human and mouse DDX3 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DDX3 antibody NB200-194 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Immunohistochemistry: DDX3 Antibody [NB200-194]

Immunohistochemistry: DDX3 Antibody [NB200-194]

Immunohistochemistry: DDX3 Antibody [NB200-194] - Detection of human DDX3 by immunohistochemistry. Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti-DDX3 (NB200-194 lot 2) used at a dilution of 1:200 (1ug/ml). Detection: DAB.
Western Blot: DDX3 Antibody [NB200-194]

Western Blot: DDX3 Antibody [NB200-194]

Western Blot: DDX3 Antibody [NB200-194] - Detection of DDX3 by Western Blot and Immunoprecipitation. Samples: A. Whole cell lysate from HeLa (5, 15 and 50 mcg) and Ramos (50 mcg) cells. B. Whole cell lysate (1 mg/IP; 1/4 of IP loaded/lane) from HeLa cells. Antibodies: NB 200-194 used at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) for Western Blot and at 3 mcg/mg lysate for IP (B). DDX3 was also immunoprecipitated using NB 200-195 and NB 200-196 at 3 mcg/mg lysate. Detection: Chemiluminescence with an exposure time of 10 seconds (A and B).
Immunohistochemistry: DDX3 Antibody [NB200-194]

Immunohistochemistry: DDX3 Antibody [NB200-194]

Immunohistochemistry: DDX3 Antibody [NB200-194] - Sample: FFPE section of human lung cancer pleural effusion (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-DDX3 used at a dilution of 1:200 (1ug/ml). Detection: Red fluorescence (A120-601D4) and DAB.
Immunohistochemistry: DDX3 Antibody [NB200-194]

Immunohistochemistry: DDX3 Antibody [NB200-194]

Immunohistochemistry: DDX3 Antibody [NB200-194] - Detection of mouse DDX3 by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-DDX3 (NB200-194 lot 2) used at a dilution of 1:200 (1ug/ml). Detection: DAB.
Immunoprecipitation: DDX3 Antibody [NB200-194]

Immunoprecipitation: DDX3 Antibody [NB200-194]

Immunoprecipitation: DDX3 Antibody [NB200-194] - Detection of human DDX3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DDX3 antibody NB200-194 (lot NB200-194-2) used for IP at 6 ug per reaction. DDX3 was also immunoprecipitated by a previous lot of this antibody (lot NB200-194-1) and rabbit anti-DDX3 antibody NB200-196. For blotting immunoprecipitated DDX3, NB200-194 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 seconds.

Applications for DDX3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:1000-1:10000
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DDX3

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which interacts specifically with hepatitis C virus core protein resulting a change in intracellular location. This gene has a homolog located in the nonrecombining region of the Y chromosome. The protein sequence is 91% identical between this gene and the Y-linked homolog. Alternative splicing of this gene generates 2 transcript variants, which are divergent in the 3' UTR.

Alternate Names

DBXCAP-Rf, DDX14, DDX3DEAD/H box-3, DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked, DEAD box protein 3, X-chromosomal, DEAD box, X isoform, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3, EC 3.6.1, EC 3.6.4.13, helicase like protein 2, Helicase-like protein 2, HLP2ATP-dependent RNA helicase DDX3X

Entrez Gene IDs

1654 (Human)

Gene Symbol

DDX3X

UniProt

NP_001347.2 (Human)

Additional DDX3 Products

Product Documents for DDX3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for DDX3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for DDX3 Antibody

There are currently no reviews for this product. Be the first to review DDX3 Antibody and earn rewards!

Have you used DDX3 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies