DDX41 Antibody (2F4) - Azide and BSA Free

Novus Biologicals | Catalog # H00051428-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2F4

Format

Azide and BSA Free
View all DDX41 Primary Antibodies »

Product Specifications

Immunogen

DDX41 (NP_057306, 523 a.a. ~ 622 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TGRSGNTGIATTFINKACDESVLMDLKALLLEAKQKVPPVLQVLHCGDESMLDIGGERGCAFCGGLGHRITDCPKLEAMQTKQVSNIGRKDYLAHSSMDF

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 23142775)

Specificity

DDX41 - DEAD (Asp-Glu-Ala-Asp) box polypeptide 41

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for DDX41 Antibody (2F4) - Azide and BSA Free

Western Blot: DDX41 Antibody (2F4) [H00051428-M01]

Western Blot: DDX41 Antibody (2F4) [H00051428-M01]

Western Blot: DDX41 Antibody (2F4) [H00051428-M01] - DDX41 monoclonal antibody (M01), clone 2F4 Analysis of DDX41 expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: DDX41 Antibody (2F4) [H00051428-M01]

Immunocytochemistry/ Immunofluorescence: DDX41 Antibody (2F4) [H00051428-M01]

Immunocytochemistry/Immunofluorescence: DDX41 Antibody (2F4) [H00051428-M01] - Analysis of monoclonal antibody to DDX41 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: DDX41 Antibody (2F4) [H00051428-M01]

Western Blot: DDX41 Antibody (2F4) [H00051428-M01]

Western Blot: DDX41 Antibody (2F4) [H00051428-M01] - Analysis of DDX41 expression in transfected 293T cell line by DDX41 monoclonal antibody (M01), clone 2F4.Lane 1: DDX41 transfected lysate(70 KDa).Lane 2: Non-transfected lysate.
ELISA: DDX41 Antibody (2F4) [H00051428-M01]

ELISA: DDX41 Antibody (2F4) [H00051428-M01]

ELISA: DDX41 Antibody (2F4) [H00051428-M01] - Detection limit for recombinant GST tagged DDX41 is approximately 0.3ng/ml as a capture antibody.

Applications for DDX41 Antibody (2F4) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate, transfected lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Reviewed Applications

Read 1 review rated 4 using H00051428-M01 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DDX41

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The function of this member has not been determined. Based on studies in Drosophila, the abstrakt gene is widely required during post-transcriptional gene expression.

Alternate Names

ABSDEAD-box protein abstrakt, DEAD (Asp-Glu-Ala-Asp) box polypeptide 41, DEAD box protein 41, DEAD box protein abstrakt homolog, EC 3.6.1, EC 3.6.4.13,2900024F02Rik, MGC8828, probable ATP-dependent RNA helicase DDX41, putative RNA helicase

Entrez Gene IDs

51428 (Human)

Gene Symbol

DDX41

OMIM

608170 (Human)

UniProt

NP_057306 (Human)

Additional DDX41 Products

Product Documents for DDX41 Antibody (2F4) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for DDX41 Antibody (2F4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for DDX41 Antibody (2F4) - Azide and BSA Free

Customer Reviews for DDX41 Antibody (2F4) - Azide and BSA Free (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: HEK CELL LYSATE
    Species: Human
    Verified Customer | Posted 02/11/2015
    DDX41 WESTERN
    DDX41 Antibody (2F4) - Azide and BSA Free H00051428-M01

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