DDX47 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85076

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (94%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DDX47 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: IHRVGRTARAGRSGKAITFVTQYDVELFQRIEHLIGKKLPGFPTQDDEVMMLTERVAEAQRFARMELREHGEKKKRSREDAGDNDDTEGAIGVRNKVAGGKMKKRKGR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DDX47 Antibody - BSA Free

Western Blot: DDX47 Antibody [NBP1-85076]

Western Blot: DDX47 Antibody [NBP1-85076]

Western Blot: DDX47 Antibody [NBP1-85076] - Analysis in human cell line NTERA-2.
Immunocytochemistry/ Immunofluorescence: DDX47 Antibody [NBP1-85076]

Immunocytochemistry/ Immunofluorescence: DDX47 Antibody [NBP1-85076]

Immunocytochemistry/Immunofluorescence: DDX47 Antibody [NBP1-85076] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli.
Immunohistochemistry-Paraffin: DDX47 Antibody [NBP1-85076]

Immunohistochemistry-Paraffin: DDX47 Antibody [NBP1-85076]

Immunohistochemistry-Paraffin: DDX47 Antibody [NBP1-85076] - Staining of human rectum shows moderate nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: DDX47 Antibody [NBP1-85076]

Immunohistochemistry-Paraffin: DDX47 Antibody [NBP1-85076]

Immunohistochemistry-Paraffin: DDX47 Antibody [NBP1-85076] - Staining of human skin shows strong nuclear positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: DDX47 Antibody [NBP1-85076]

Immunohistochemistry-Paraffin: DDX47 Antibody [NBP1-85076]

Immunohistochemistry-Paraffin: DDX47 Antibody [NBP1-85076] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.
Immunohistochemistry-Paraffin: DDX47 Antibody [NBP1-85076]

Immunohistochemistry-Paraffin: DDX47 Antibody [NBP1-85076]

Immunohistochemistry-Paraffin: DDX47 Antibody [NBP1-85076] - Staining of human tonsil shows strong nuclear positivity in germinal center cells.

Applications for DDX47 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DDX47

DDX47 encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene can shuttle between the nucleus and the cytoplasm, and has an RNA-independent ATPase activity. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Alternate Names

DEAD (Asp-Glu-Ala-Asp) box polypeptide 47, DEAD box polypeptide 47, DEAD box protein 47, DKFZp564O176, E4-DBP, E4-DEAD box protein, EC 3.6.1, EC 3.6.4.13, FLJ30012, HQ0256, MSTP162, probable ATP-dependent RNA helicase DDX47, RRP3

Gene Symbol

DDX47

Additional DDX47 Products

Product Documents for DDX47 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for DDX47 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for DDX47 Antibody - BSA Free

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Protocols

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FAQs

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