DDX5 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-30944

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Primate (100%), Rat (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DDX5 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human DDX5. The exact sequence is proprietary.

Localization

Nucleus, nucleolus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DDX5 Antibody - BSA Free

Western Blot: DDX5 Antibody [NBP1-30944]

Western Blot: DDX5 Antibody [NBP1-30944]

Western Blot: DDX5 Antibody [NBP1-30944] - Human lymphocytes. Antibody 1:500 dilution in milk. 6 minute exposure. ~25kDA band shows up as strong as the expected band at ~75kDA. This image was submitted via customer review.
Immunocytochemistry/ Immunofluorescence: DDX5 Antibody [NBP1-30944]

Immunocytochemistry/ Immunofluorescence: DDX5 Antibody [NBP1-30944]

Immunocytochemistry/Immunofluorescence: DDX5 Antibody [NBP1-30944] - Paraformaldehyde-fixed A431, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: DDX5 Antibody [NBP1-30944]

Immunohistochemistry-Paraffin: DDX5 Antibody [NBP1-30944]

Immunohistochemistry-Paraffin: DDX5 Antibody [NBP1-30944] - Paraffin-embedded lung SCC xenograft, using antibody at 1:100 dilution.
Western Blot: DDX5 Antibody [NBP1-30944]

Western Blot: DDX5 Antibody [NBP1-30944]

Western Blot: DDX5 Antibody [NBP1-30944] - 30 ug of whole cell lysate, H1299 7. 5% SDS-PAGE DDX5. Antibody at 1:1000.

Applications for DDX5 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:10 - 1:500

Western Blot

1:500-1:3000

Reviewed Applications

Read 3 reviews rated 3.3 using NBP1-30944 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DDX5

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified.

Long Name

DEAD Box Protein 5

Alternate Names

DEAD/H, G17P1, HELR, HLR1, HUMP68

Gene Symbol

DDX5

Additional DDX5 Products

Product Documents for DDX5 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for DDX5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Customer Reviews for DDX5 Antibody - BSA Free (3)

3.3 out of 5
3 Customer Ratings
5 Stars
0%
4 Stars
33%
3 Stars
67%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • DDX5 Antibody
    Name: Anonymous
    Application: Immunoprecipitation
    Sample Tested: B-cell
    Species: Human
    Verified Customer | Posted 01/02/2019
    DDX5 immunoprecipitation in human B-cells.
    10ug antibody used with 500ug human B-cell whole cell lysate. L1: 40ug input, L2: IgG, L3: IP (10ul of 50ul elution)
    DDX5 Antibody - BSA Free NBP1-30944
  • DDX5 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Lymphocytes
    Species: Human
    Verified Customer | Posted 03/13/2018
    1:500 dilution in milk. 6 minute exposure. ~25kDA band shows up as strong as the expected band at ~75kDA.
    BIOSCARF
    DDX5 Antibody - BSA Free NBP1-30944
  • DDX5 Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: fibroblasts
    Species: Human
    Verified Customer | Posted 01/11/2018
    1:500 in 1X PBS/1% BSA/0.3% Trito X-100, 16 hours, 4C.
    DDX5 Antibody - BSA Free NBP1-30944

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Protocols

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FAQs

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