DDX51 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85859

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DDX51 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GFGKRKAPKVQPFLPRWLAEPNCVRRNVTEDLVPIEDIPDVHPDLQKQLRAHGISSYFPVQAAVIPALLESAACGFLVGRGGYRPSDLCVSA

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%), Rat (82%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DDX51 Antibody - BSA Free

Immunohistochemistry-Paraffin: DDX51 Antibody [NBP1-85859]

Immunohistochemistry-Paraffin: DDX51 Antibody [NBP1-85859]

Immunohistochemistry-Paraffin: DDX51 Antibody [NBP1-85859] - Staining of human stomach, upper shows moderate cytoplasmic positivity in tubule cells.
DDX51 Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: DDX51 Antibody - BSA Free [NBP1-85859]

Immunocytochemistry/ Immunofluorescence: DDX51 Antibody - BSA Free [NBP1-85859]

Staining of human cell line A-431 shows localization to nucleus, nucleoli & cytosol.
DDX51 Antibody - BSA Free Western Blot: DDX51 Antibody - BSA Free [NBP1-85859]

Western Blot: DDX51 Antibody - BSA Free [NBP1-85859]

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp

Applications for DDX51 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DDX51

DDX51 is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. DDX51 may function in biogenesis of the 60S ribosomal subunit. Alternate names for DDX51 include ATP-dependent RNA helicase DDX51, DEAD box protein 51, MGC42193, and DKFZp686N2081.

Alternate Names

ATP-dependent RNA helicase DDX51, DEAD (Asp-Glu-Ala-Asp) box polypeptide 51, DEAD box protein 51, DKFZp686N2081, EC 3.6.1, EC 3.6.4.13, MGC42193

Gene Symbol

DDX51

Additional DDX51 Products

Product Documents for DDX51 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for DDX51 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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